This is the list of the number ones of the Official Subscription Plays Chart during the 2000s. The first song to top the chart was "I Kissed a Girl" by Katy Perry.
| scope=col | Artist | scope=col | Song | scope=col | Record label | scope=col | Reached number one (for the week ending) | scope=col | Weeks at number one |
|---|---|---|---|---|---|---|---|---|---|
| scope=row style="text-align:center;" colspan=6 | |||||||||
| featuring Akon | "Sexy Chick" | Positiva/Virgin | 2 | ||||||
| "I Gotta Feeling" | Interscope | 1 | |||||||
| Shakira | "She Wolf" | Epic | 1 | ||||||
| featuring Flo Rida | "Bad Boys" | Syco | 1 | ||||||
| "Fight for This Love" | Fascination | 3 | |||||||
| "Meet Me Halfway" | Interscope | 1 | |||||||
| "Fight for This Love" | Fascination | 1 | |||||||
| "Meet Me Halfway" | Interscope | 1 | |||||||
| Lady Gaga | "Bad Romance" | Interscope | 2 |