This is a list of National Collegiate Athletic Association (NCAA) schools in the United States and Canada that play lacrosse as a varsity sport at the Division II level. In the 2024 NCAA lacrosse season, there are 77 men's and 121 women's Division II lacrosse programs.
Dates of addition or dropping of lacrosse, or changes in conference affiliation, reflect the time at which the change takes effect. Because NCAA lacrosse for both men and women is a spring sport, this will not match the first overall season of competition for new teams, or the first season of competition in a new conference. For teams departing Division II, the departure will take place after the completion of that year's lacrosse season.
Conference affiliations are current for the upcoming 2025 NCAA lacrosse season.
| School | Nickname | City | State/ province | Future conference | Note | (M) | (W) | Begins play |
|---|---|---|---|---|---|---|---|---|
| Ferrum College | Panthers | Ferrum | Virginia | Conference Carolinas | 2026 | |||
| Nova Southeastern University | Sharks | Fort Lauderdale | Florida | Sunshine State Conference |