Conference affiliations reflect those in the 2025 season. Years of conference changes, indicated in footnotes, reflect softball seasons, which take place in the calendar year after a conference change takes effect.
| School | Nickname | State | Home field | Conference | National titles |
|---|
| Central Baptist College | Mustangs | Arkansas | City of Colleges Park | American Midwest | None |
| Columbia College | Cougars | Missouri | Antimi Softball Complex | American Midwest | None |
| Cottey College | Comets | Missouri | Vanek Family Softball Field | American Midwest | None |
| Hannibal–LaGrange University | Trojans | Missouri | HLGU Field | American Midwest | None |
| Harris–Stowe State University | Hornets | Missouri | Stars Park | American Midwest | None |
| Mission University | Patriots | Missouri | Various | American Midwest | None |
| Missouri Baptist University | Spartans | Missouri | Spartan Softball Field | American Midwest | None |
| University of Health Sciences and Pharmacy in St. Louis | Eutectics | Missouri | Affton Athletic Association | American Midwest | None |
| Stephens College | Stars | Missouri | Battle High School Softball Field | American Midwest | None |
| William Woods University | Owls | Missouri | Backer Sports Complex | American Midwest | None |
| Williams Baptist University | Eagles | Arkansas | WBU Softball Field | American Midwest | None |
| Bluefield University | Rams | Virginia | Graham Rec Park | Appalachian Athletic | None |
| Brenau University | Golden Tigers | Georgia | Ernest Ledford Grindle Athletics Park | Appalachian Athletic | None |
| Bryan College | Lions | Tennessee | BC Softball Field | Appalachian Athletic | None |
| Columbia College | Koalas | South Carolina | Sutton Softball Complex | Appalachian Athletic | None |
| Columbia International University | Rams | South Carolina | CIU Softball Field | Appalachian Athletic | None |
| Johnson University | Royals | Tennessee | Johnson University Softball Field | Appalachian Athletic | None |
| Kentucky Christian University | Knights | Kentucky | Kentucky Christian Softball Field | Appalachian Athletic | None |
| Milligan University | Buffaloes | Tennessee | Anglin Field | Appalachian Athletic | None |
| Montreat College | Cavaliers | North Carolina | Roxy Hines Memorial Softball Field | Appalachian Athletic | None |
| University of Pikeville | Bears | Kentucky | Paul Butcher Field | Appalachian Athletic | None |
| Reinhardt University | Eagles | Georgia | Ken White Softball Field | Appalachian Athletic | None |
| St. Andrews University | Knights | North Carolina | Knights Softball Field | Appalachian Athletic | None |
| Tennessee Wesleyan University | Bulldogs | Tennessee | Athens Regional Park | Appalachian Athletic | None |
| Truett McConnell University | Bears | Georgia | TMU Softball Complex | Appalachian Athletic | None |
| Union Commonwealth University | Lady Bulldogs | Kentucky | Union Field | Appalachian Athletic | None |
| La Sierra University | Golden Eagles | California | La Sierra Softball Field | California Pacific | None |
| Simpson University | Red Hawks | California | Simpson Softball Field | California Pacific | None |
| Westcliff University | Warriors | California | OC Great Park | California Pacific | None |
| University of British Columbia | Thunderbirds | British Columbia | Collings Field at Nobel Park | Cascade Collegiate | None |
| Bushnell University | Beacons | Oregon | Rich Schwab Field | Cascade Collegiate | None |
| Carroll College | Fighting Saints | Montana | Centennial Park | Cascade Collegiate | None |
| Corban University | Warriors | Oregon | Warrior Softball Field | Cascade Collegiate | None |
| Eastern Oregon University | Mountaineers | Oregon | Peggy Anderson Softball Field | Cascade Collegiate | None |
| College of Idaho | Coyotes | Idaho | Symms Field | Cascade Collegiate | None |
| Northwest University | Eagles | Washington | Bellevue College Softball Field | Cascade Collegiate | None |
| Oregon Institute of Technology | Owls | Oregon | John and Lois Stilwell Stadium | Cascade Collegiate | 2011 |
| University of Providence | Argonauts | Montana | University of Providence Softball Field | Cascade Collegiate | None |
| Southern Oregon University | Raiders | Oregon | University Softball Field | Cascade Collegiate | 2019, 2021 |
| Warner Pacific University | Knights | Oregon | Erv Lind Stadium | Cascade Collegiate | None |
| Calumet College of St. Joseph | Crimson Wave | Indiana | Whiting Sports Complex | Chicagoland Collegiate Athletic | None |
| Indiana University South Bend | Titans | Indiana | Dick Pfeil Softball Field | Chicagoland Collegiate Athletic | None |
| Judson University | Eagles | Illinois | Judson Softball Complex | Chicagoland Collegiate Athletic | None |
| Olivet Nazarene University | Tigers | Illinois | Tiger Field | Chicagoland Collegiate Athletic | None |
| St. Ambrose University | Fighting Bees | Iowa | St. Vincent's Athletic Complex | Chicagoland Collegiate Athletic | None |
| University of St. Francis | Fighting Saints | Illinois | Harlow Lockwood Field | Chicagoland Collegiate Athletic | None |
| Saint Xavier University | Cougars | Illinois | SXU Softball Field | Chicagoland Collegiate Athletic | None |
| Trinity Christian College | Trolls | Illinois | Schaaf Athletics Complex | Chicagoland Collegiate Athletic | None |
| Viterbo University | V-Hawks | Wisconsin | Viterbo Outdoor Athletics Complex | Chicagoland Collegiate Athletic | None |
| Bethel University | Pilots | Indiana | Gardner Field | Crossroads League | None |
| Goshen College | Maple Leafs | Indiana | Goshen College Softball Complex | Crossroads League | None |
| Grace College & Seminary | Lancers | Indiana | Coach Phil Dick Field | Crossroads League | None |
| Huntington University | Foresters | Indiana | HU Softball Field | Crossroads League | None |
| Indiana Wesleyan University | Wildcats | Indiana | Sue Bowman Field | Crossroads League | None |
| Marian University | Knights | Indiana | MU Softball Field | Crossroads League | None |
| Mount Vernon Nazarene University | Cougars | Ohio | Cougar Softball Field | Crossroads League | None |
| University of Saint Francis | Cougars | Indiana | Westendorf Field | Crossroads League | None |
| Spring Arbor University | Cougars | Michigan | Cougar Softball Field | Crossroads League | None |
| Taylor University | Trojans | Indiana | Gudakunst Field | Crossroads League | None |
| Arizona Christian University | Firestorm | Arizona | ACU Softball Field | Golden State Athletic | None |
| Benedictine University–Mesa | Redhawks | Arizona | Seton Catholic Prep Softball Field | Golden State Athletic | None |
| Embry–Riddle Aeronautical University, Prescott | Eagles | Arizona | Eagle Softball Field | Golden State Athletic | None |
| Hope International University | Royals | California | Bastanchury Park | Golden State Athletic | None |
| Ottawa University | Spirit | Arizona | Willow Canyon HS Softball Field | Golden State Athletic | None |
| Park University–Gilbert | Buccaneers | Arizona | Chandler–Gilbert Community College Softball Field | Golden State Athletic | None |
| San Diego Christian College | Hawks | California | Santee Sportsplex | Golden State Athletic | None |
| Briar Cliff University | Chargers | Iowa | Bishop Mueller Field | Great Plains Athletic | None |
| Concordia University Nebraska | Bulldogs | Nebraska | Plum Creek Park | Great Plains Athletic | None |
| Dakota Wesleyan University | Tigers | South Dakota | Cadwell Park | Great Plains Athletic | None |
| Doane University | Tigers | Nebraska | Doane Softball Field | Great Plains Athletic | None |
| Dordt University | Defenders | Iowa | Dordt Softball Complex | Great Plains Athletic | None |
| Hastings College | Broncos | Nebraska | Smith Softball Complex | Great Plains Athletic | None |
| Midland University | Warriors | Nebraska | Christensen Field | Great Plains Athletic | None |
| Morningside University | Mustangs | Iowa | Jensen Softball Complex | Great Plains Athletic | None |
| Mount Marty University | Lancers | South Dakota | North-East Sertoma Fields | Great Plains Athletic | None |
| Northwestern College | Red Raiders | Iowa | Northwestern Softball Diamond | Great Plains Athletic | None |
| College of Saint Mary | Flames | Nebraska | Saint Mary Softball Field | Great Plains Athletic | None |
| Baker University | Wildcats | Kansas | Cavaness Field | Heart of America Athletic | None |
| Benedictine College | Ravens | Kansas | Benedictine Softball Field | Heart of America Athletic | None |
| Central Methodist University | Eagles | Missouri | Cox Softball Complex | Heart of America Athletic | None |
| Clarke University | Pride | Iowa | Veterans Memorial Park | Heart of America Athletic | None |
| Culver–Stockton College | Wildcats | Missouri | Himsl Field | Heart of America Athletic | None |
| Graceland University | Yellowjackets | Iowa | Graceland Softball Field | Heart of America Athletic | None |
| Grand View University | Vikings | Iowa | Busse Field | Heart of America Athletic | None |
| MidAmerica Nazarene University | Pioneers | Kansas | Williams Field | Heart of America Athletic | None |
| Missouri Valley College | Vikings | Missouri | Indian Foothills Park/Lyon Bowl | Heart of America Athletic | None |
| Mount Mercy University | Mustangs | Iowa | Robert W. Plaster Athletic Complex | Heart of America Athletic | None |
| Park University | Pirates | Missouri | Park University Softball Complex | Heart of America Athletic | None |
| Peru State College | Bobcats | Nebraska | Peru State Softball Field | Heart of America Athletic | None |
| Waldorf University | Warriors | Iowa | Al Buckley Field | Great Plains Athletic | None |
| William Penn University | Lady Statesmen | Iowa | Penn Softball Field | Heart of America Athletic | None |
| Arkansas Baptist College | Buffaloes | Arkansas | Arkansas Baptist Softball Field | Independent | None |
| Bacone College | Warriors | Oklahoma | Claude Cox Omniplex | Independent | None |
| Carolina University | Bruins | North Carolina | Wilson Park | Independent | None |
| Crowley's Ridge College | Pioneers | Arkansas | Pioneer Park | Independent | None |
| Fisher College | Falcons | Massachusetts | Daly Field | Independent | None |
| Florida National University | Conquistadors | Florida | Florida National Softball Field | Independent | None |
| Georgia Gwinnett College | Grizzlies | Georgia | Grizzly Softball Complex | Independent | None |
| Haskell Indian Nations University | Fighting Indians | Kansas | Coffin Sports Complex Softball Field | Independent | None |
| Indiana University Columbus | Crimson Pride | Indiana | Deaconess Sports Park | Independent | None |
| Morris College | Hornets | South Carolina | Morris College Softball Field | Independent | None |
| Penn State Schuylkill | Nittany Lions | Pennsylvania | The Island Complex | Independent | None |
| Rust College | Bearcats | Mississippi | Rust College Softball Field | Independent | None |
| Spartanburg Methodist College | Pioneers | South Carolina | Grant Softball Field | Independent | NOne |
| Talladega College | Tornadoes | Alabama | Ben E. Bruner Complex | Independent | None |
| Voorhees University | Lady Tigers | South Carolina | Voorhees Softball Field | Independent | None |
| Washington Adventist University | Shock | Maryland | Washington Adventist Softball Field | Independent | None |
| Avila University | Eagles | Missouri | The Z | Kansas Collegiate Athletic | None |
| Bethany College | Swedes | Kansas | Swedes Softball Field | Kansas Collegiate Athletic | None |
| Bethel College | Threshers | Kansas | Wedel Field | Kansas Collegiate Athletic | None |
| Evangel University | Valor | Missouri | Evangel Softball Complex | Kansas Collegiate Athletic | None |
| Friends University | Falcons | Kansas | Two Rivers Youth Complex | Kansas Collegiate Athletic | None |
| Kansas Wesleyan University | Coyotes | Kansas | Salina South High School Softball Field | Kansas Collegiate Athletic | None |
| McPherson College | Bulldogs | Kansas | Bulldog Park | Kansas Collegiate Athletic | None |
| Oklahoma Wesleyan University | Eagles | Oklahoma | Oklahoma Wesleyan University Softball Complex | Kansas Collegiate Athletic | None |
| Ottawa University | Braves | Kansas | Dick Peters Sports Complex | Kansas Collegiate Athletic | None |
| University of Saint Mary | Spires | Kansas | University of Saint Mary Softball Complex | Kansas Collegiate Athletic | None |
| Southwestern College | Moundbuilders | Kansas | Broadway Sports Complex | Kansas Collegiate Athletic | None |
| Sterling College | Warriors | Kansas | Sterling High School Softball Field | Kansas Collegiate Athletic | None |
| Tabor College | Bluejays | Kansas | Hillsboro Sports Complex | Kansas Collegiate Athletic | None |
| York University | Panthers | Nebraska | York Ballpark Complex | Kansas Collegiate Athletic | None |
| Bethel University | Wildcats | Tennessee | Lady Wildcat Park at the Kahlden Complex | Mid-South | None |
| Campbellsville University | Tigers | Kentucky | Hord Softball Field | Mid-South | None |
| Cumberland University | Phoenix | Tennessee | Billy Dee Ross Stadium | Mid-South | None |
| University of the Cumberlands | Patriots | Kentucky | UC Softball Complex | Mid-South | None |
| Freed–Hardeman University | Lions | Tennessee | Morgan Stadium at Tucker Field | Mid-South | None |
| Georgetown College | Tigers | Kentucky | Tiger Softball Complex | Mid-South | None |
| Lindsey Wilson College | Blue Raiders | Kentucky | Marilyn D. Sparks Park | Mid-South | None |
| Bellevue University | Bruins | Nebraska | Roy Smith Field | NSAA | None |
| Dakota State University | Trojans | South Dakota | Thue Softball Complex | NSAA | None |
| Dickinson State University | Blue Hawks | North Dakota | North Softball Complex | NSAA | None |
| University of Jamestown | Jimmies | North Dakota | Trapper Field | NSAA | None |
| Mayville State University | Comets | North Dakota | Paul Karlstad Field | NSAA | None |
| Valley City State University | Vikings | North Dakota | VCSU Softball Complex | NSAA | None |
| University of Houston–Victoria | Jaguars | Texas | City of Victoria Youth Sports Complex | Red River Athletic | None |
| Huston–Tillotson University | Rams | Texas | Krieg Fields | Red River Athletic | None |
| Jarvis Christian University | Bulldogs | Texas | Lady Bulldog Softball Field | Red River Athletic | None |
| Louisiana Christian University | Lady Wildcats | Louisiana | Wildcat Park | Red River Athletic | None |
| Louisiana State University of Alexandria | Generals | Louisiana | Generals Softball Field | Red River Athletic | None |
| Our Lady of the Lake University | Saints | Texas | Our Lady of the Lake University Softball Field | Red River Athletic | None |
| University of the Southwest | Mustangs | New Mexico | Baker Field | Red River Athletic | None |
| Texas College | Steers | Texas | Texas College Softball Field | Red River Athletic | None |
| Texas A&M University–San Antonio | Jaguars | Texas | Tejeda Sports Complex | Red River Athletic | None |
| Texas A&M University–Texarkana | Eagles | Texas | Bramlett Field | Red River Athletic | None |
| Xavier University of Louisiana | Gold Nuggets | Louisiana | Wesley Barrow Stadium | Red River Athletic | None |
| Alice Lloyd College | Eagles | Kentucky | Alice Lloyd Softball Field | River States | None |
| Brescia University | Bearcats | Kentucky | Kamuf Park | River States | None |
| Indiana University Southeast | Grenadiers | Indiana | Koetter Sports Softball Complex | River States | None |
| Midway University | Eagles | Kentucky | Clark Memorial Softball Field | River States | None |
| Oakland City University | Mighty Oaks | Indiana | EGGSL Field | River States | None |
| University of Rio Grande | RedStorm | Ohio | Stanley L. Evans Memorial Athletic Complex | River States | None |
| Saint Mary-of-the-Woods College | Pomeroys | Indiana | SMWC Softball Field | River States | None |
| Shawnee State University | Bears | Ohio | Dr. Singleton Park | River States | None |
| West Virginia University Institute of Technology | Golden Bears | West Virginia | Fitzpatrick Park | River States | None |
| Central Christian College of Kansas | Tigers | Kansas | Central Christian College of Kansas Softball Complex | Sooner Athletic | None |
| Langston University | Lions | Oklahoma | T.G. Green Park Softball Field | Sooner Athletic | None |
| Mid-America Christian University | Evangels | Oklahoma | Allen Field | Sooner Athletic | None |
| Nelson University | Lions | Texas | Nelson Softball Field | Sooner Athletic | None |
| Oklahoma City University | Stars | Oklahoma | Ann Lacy Stadium | Sooner Athletic | 1994, 1995, 1996, 1997, 2000, 2001, 2002, 2007, 2016, 2017, 2022 |
| Oklahoma Panhandle State University | Aggies | Oklahoma | Jim Quimby Field | Sooner Athletic | None |
| University of Science and Arts of Oklahoma | Drovers | Oklahoma | Bill Smith Ballpark Softball | Sooner Athletic | 2018 |
| Southwestern Christian University | Eagles | Oklahoma | SCU Softball Field | Sooner Athletic | None |
| Texas Wesleyan University | Rams | Texas | Sycamore Park | Sooner Athletic | None |
| Wayland Baptist University | Pioneers | Texas | Running Water Draw Regional Park Softball Field | Sooner Athletic | None |
| Abraham Baldwin Agricultural College | Golden Stallions | Georgia | Field at Red Hill Athletic Complex | Southern States Athletic | None |
| Blue Mountain Christian University | Toppers | Mississippi | BMC Sportsplex | Southern States Athletic | None |
| Brewton–Parker College | Barons | Georgia | BPC Softball Field | Southern States Athletic | None |
| Faulkner University | Eagles | Alabama | Wynn Field | Southern States Athletic | None |
| Middle Georgia State University | Knights | Georgia | Knights Field | Southern States Athletic | None |
| University of Mobile | Rams | Alabama | UM Softball Field | Southern States Athletic | 2006 |
| Point University | Skyhawks | Georgia | West Point Park Softball Field | Southern States Athletic | None |
| Stillman College | Tigers | Alabama | Stillman Softball Complex | Southern States Athletic | None |
| Thomas University | Night Hawks | Georgia | Armory Field | Southern States Athletic | 2004 |
| University of Tennessee Southern | FireHawks | Tennessee | Royce Hughes Field | Southern States Athletic | None |
| William Carey University | Crusaders | Mississippi | Joseph & Nancy Fail Field | Southern States Athletic | None |
| Ave Maria University | Gyrenes | Florida | Ave Maria Softball Field | Sun Conference | None |
| College of Coastal Georgia | Mariners | Georgia | Coastal Georgia Softball Field | Sun Conference | None |
| Florida Memorial University | Lions | Florida | Carol City Park | Sun Conference | None |
| Keiser University | Seahawks | Florida | Alumni Field | Sun Conference | None |
| St. Thomas University | Bobcats | Florida | Col. Jacquelin J. Kelly Field | Sun Conference | None |
| Southeastern University | Fire | Florida | SEU Softball Field | Sun Conference | None |
| Warner University | Royals | Florida | Daly Field | Sun Conference | None |
| Webber International University | Warriors | Florida | Nancy Nichols Field | Sun Conference | None |
| Aquinas College | Saints | Michigan | Gainey Athletic Complex | Wolverine–Hoosier Athletic | None |
| Cleary University | Cougars | Michigan | Lake Trust Stadium | Wolverine–Hoosier Athletic | None |
| Concordia University Ann Arbor | Cardinals | Michigan | Cardinal Softball Field | Wolverine–Hoosier Athletic | None |
| Cornerstone University | Golden Eagles | Michigan | CU Softball Field | Wolverine–Hoosier Athletic | None |
| Defiance College | Lady Jackets | Ohio | Sal Hench Field | Wolverine–Hoosier Athletic | None |
| Indiana Institute of Technology | Warriors | Indiana | Warrior Park | Wolverine–Hoosier Athletic | None |
| Lawrence Technological University | Blue Devils | Michigan | Buysse Ballpark | Wolverine–Hoosier Athletic | None |
| Lourdes University | Gray Wolves | Ohio | Gray Wolf Field | Wolverine–Hoosier Athletic | None |
| Madonna University | Crusaders | Michigan | University Field | Wolverine–Hoosier Athletic | None |
| University of Michigan–Dearborn | Wolverines | Michigan | Gary L. Bryce Field | Wolverine–Hoosier Athletic | None |
| University of Northwestern Ohio | Racers | Ohio | Racer Softball Field | Wolverine–Hoosier Athletic | None |
| Rochester Christian University | Warriors | Michigan | Jaycee Field | Wolverine–Hoosier Athletic | None |
| Siena Heights University | Saints | Michigan | Christensen Memorial Softball Field | Wolverine–Hoosier Athletic | None | |
