This is a list of the Dutch Top 40 number-one singles of 2016. The Dutch Top 40 is a chart that ranks the best-performing singles of the Netherlands. It is published every week by radio station Radio 538.
| Issue date | Song | Artist(s) | Ref. |
|---|---|---|---|
| January 2 | "Sorry" | Justin Bieber | [1] |
| January 9 | "Love Yourself" | [2] | |
| January 16 | [3] | ||
| January 23 | [4] | ||
| January 30 | "7 Years" | Lukas Graham | [5] |
| February 6 | [6] | ||
| February 13 | "I Took a Pill in Ibiza" (SeeB remix) | Mike Posner | [7] |
| February 20 | [8] | ||
| February 27 | [9] | ||
| March 5 | [10] | ||
| March 12 | [11] | ||
| March 19 | [12] | ||
| March 26 | [13] | ||
| April 2 | [14] | ||
| April 9 | [15] | ||
| April 16 | [16] | ||
| April 23 | "Work from Home" | Fifth Harmony featuring Ty Dolla Sign | [17] |
| April 30 | "One Dance" | Drake featuring Wizkid and Kyla | [18] |
| May 7 | [19] | ||
| May 14 | [20] | ||
| May 21 | [21] | ||
| May 28 | [22] | ||
| June 4 | [23] | ||
| June 11 | "Can't Stop the Feeling!" | Justin Timberlake | [24] |
| June 18 | [25] | ||
| June 25 | [26] | ||
| July 2 | [27] | ||
| July 9 | [28] | ||
| July 16 | [29] | ||
| July 23 | [30] | ||
| July 30 | [31] | ||
| August 6 | "Cold Water" | Major Lazer featuring Justin Bieber and MØ | [32] |
| August 13 | [33] | ||
| August 20 | [34] | ||
| August 27 | [35] | ||
| September 3 | [36] | ||
| September 10 | [37] | ||
| September 17 | "Let Me Love You" | DJ Snake featuring Justin Bieber | [38] |
| September 24 | [39] | ||
| October 1 | [40] | ||
| October 8 | [41] | ||
| October 15 | [42] | ||
| October 22 | "Closer" | The Chainsmokers featuring Halsey | [43] |
| October 29 | [44] | ||
| November 5 | [45] | ||
| November 12 | [46] | ||
| November 19 | "Starboy" | The Weeknd featuring Daft Punk | [47] |
| November 26 | [48] | ||
| December 2 | [49] | ||
| December 9 | [50] | ||
| December 16 | "Rockabye" | Clean Bandit featuring Sean Paul and Anne-Marie | [51] |
| December 23 | [52] | ||
| December 30 | [53] | ||
| Position | Artist | Weeks #1 | |
|---|---|---|---|
| 1 | 15 | ||
| 2 | 10 | ||
| 2 | SeeB (as featuring) | 10 | |
| 3 | 8 | ||
| 4 | 6 | ||
| 4 | Wizkid (as featuring) | 6 | |
| 4 | Kyla (as featuring) | 6 | |
| 4 | 6 | ||
| 4 | MØ (as featuring) | 6 | |
| 5 | 5 | ||
| 6 | 4 | ||
| 6 | Halsey (as featuring) | 4 | |
| 6 | 4 | ||
| 6 | Daft Punk (as featuring) | 4 | |
| 7 | 3 | ||
| 7 | Anne-Marie (as featuring) | 3 | |
| 7 | Sean Paul (as featuring) | 3 | |
| 8 | 2 | ||
| 9 | 1 | ||
| 9 | Ty Dolla Sign (as featuring) | 1 |