These hits topped the Dutch Top 40 in 2000 (see 2000 in music).[1]
| Issue date | Artist(s) | Song |
|---|---|---|
| 1 January | "Binnen" | |
| 8 January | ||
| 15 January | ||
| 22 January | "Drop It" | |
| 29 January | ||
| 5 February | ||
| 12 February | "Onderweg" | |
| 19 February | ||
| 26 February | ||
| 4 March | ||
| 11 March | ||
| 18 March | ||
| 25 March | "Freestyler" | |
| 1 April | ||
| 8 April | ||
| 15 April | ||
| 22 April | ||
| 29 April | "Never Be the Same Again" | |
| 6 May | ||
| 13 May | "Oops!... I Did It Again" | |
| 20 May | ||
| 27 May | "It's My Life" | |
| 3 June | ||
| 10 June | Jop | "Jij bent de zon" |
| 17 June | ||
| 24 June | ||
| 1 July | ||
| 8 July | Jody Bernal | "Que si, que no" |
| 15 July | ||
| 22 July | ||
| 29 July | ||
| 5 August | ||
| 12 August | "I Would Stay" | |
| 19 August | ||
| 26 August | ||
| 2 September | Melanie C | "I Turn to You" |
| 9 September | ||
| 16 September | ||
| 23 September | ||
| 30 September | "Could I Have This Kiss Forever" | |
| 7 October | Jody Bernal | "Que si, que no" |
| 14 October | ||
| 21 October | "Beautiful Day" | |
| 28 October | ||
| 4 November | "Wêr bisto" | |
| 11 November | ||
| 18 November | ||
| 25 November | ||
| 2 December | ||
| 9 December | ||
| 16 December | "Can't Fight the Moonlight" | |
| 23 December | ||
| 30 December |