These are the number-one singles of 1965 according to the Top 100 Singles chart in Cashbox magazine.
| Issue Date | Song | Artist | |
|---|---|---|---|
| January 2 | I Feel Fine | The Beatles | |
| January 9 | |||
| January 16 | |||
| January 23 | Downtown | Petula Clark | |
| January 30 | |||
| February 6 | You've Lost That Lovin' Feelin' | The Righteous Brothers | |
| February 13 | |||
| February 20 | |||
| February 27 | This Diamond Ring | Gary Lewis & the Playboys | |
| March 6 | Eight Days a Week | The Beatles | |
| March 13 | |||
| March 20 | |||
| March 27 | Stop! In the Name of Love | The Supremes | |
| April 3 | Can't You Hear My Heartbeat | Herman's Hermits | |
| April 10 | I'm Telling You Now | Freddie & The Dreamers | |
| April 17 | |||
| April 24 | Game of Love | Wayne Fontana & The Mindbenders | |
| May 1 | Herman's Hermits | ||
| May 8 | |||
| May 15 | |||
| May 22 | |||
| May 29 | Ticket to Ride | The Beatles | |
| June 5 | Back in My Arms Again | The Supremes | |
| June 12 | Help Me, Rhonda | The Beach Boys | |
| June 19 | The Four Tops | ||
| June 26 | |||
| July 3 | Mr. Tambourine Man | The Byrds | |
| July 10 | (I Can't Get No) Satisfaction | The Rolling Stones | |
| July 17 | |||
| July 24 | |||
| July 31 | |||
| August 7 | I'm Henry VIII, I Am | Herman's Hermits | |
| August 14 | I Got You Babe | Sonny & Cher | |
| August 21 | |||
| August 28 | Help! | The Beatles | |
| September 4 | |||
| September 11 | |||
| September 18 | Like a Rolling Stone | Bob Dylan | |
| September 25 | Eve of Destruction | Barry McGuire | |
| October 2 | Hang on Sloopy | The McCoys | |
| October 9 | Yesterday | The Beatles | |
| October 16 | |||
| October 23 | A Lover's Concerto | The Toys | |
| October 30 | Yesterday | The Beatles | |
| November 6 | Get Off of My Cloud | The Rolling Stones | |
| November 13 | |||
| November 20 | I Hear a Symphony | The Supremes | |
| November 27 | 1-2-3 | Len Barry | |
| December 4 | The Byrds | ||
| December 11 | Let's Hang On! | The 4 Seasons | |
| December 18 | Taste of Honey | Herb Alpert & The Tijuana Brass | |
| December 25 | Over and Over | The Dave Clark Five |