Before Ambush Bug received his own mini-series, he appeared in DC Comics Presents #52 and #59 as a villain, later becoming a hero in Supergirl #16. He discovered that Clark Kent is really Superman in Action Comics #560; revealed his origin in issue #563; and in issue #565, tried (and failed) to get Batman, Superman, the Teen Titans, and Wonder Woman to appear in his mini-series. In DC Comics Presents #81 (his last pre-series appearance), he switches bodies with Superman for 48 hours.
In this series, Ambush Bug battles bombers that "killed" his sidekick Cheeks in issue #1, and then he turned Quentin Quantis (in mutated form) human again in issue #2. In issue #3, he talked about lesser known characters and, in issue #4, had his showdown with Darkseid, while in the last issue, he meets Argh! Yle!
In this one-shot, Ambush Bug's toy sidekick turns into a cannibal zombie, so it is up to Ambush Bug to stop him.
In this second mini-series, Ambush Bug tries to stop the Interferer (a former comic book writer with god-like powers) before being sent to court for "contempt of comics". He is then kicked out of the DC Universe.
Despite the end of the Son of Ambush Bug series, Ambush Bug reappears in Secret Origin #48, where he refuses to reveal his origins.
See main article: Ambush Bug: Year None.
In 2009, DC Comics released a black-and-white, 478-page collection of comics featuring Ambush Bug .