The following railroads have operated in the State of Alabama.
| Name | Mark | System[1] | From | To | Successor | Notes |
|---|---|---|---|---|---|---|
| Abbeville–Grimes Railway | AG | 1989 | 1994 | A&G Railroad | ||
| Abbeville Southern Railway | ACL | 1892 | 1901 | Savannah, Florida and Western Railway | ||
| A&G Railroad | AG | 1994 | 1996 | Bay Line Railroad | ||
| Alabama Central Railroad (1906) | 1906 | 1961 | N/A | Commemorative website: http://www.alabamacentralrailroad.com/home/]]) | ||
| Alabama Central Railroad | SOU | 1871 | 1881 | East Tennessee, Virginia and Georgia Railroad | ||
| Alabama Central Railway | 1903 | 1939 | N/A | |||
| Alabama and Chattanooga Railroad | SOU | 1868 | 1877 | Alabama Great Southern Railroad | ||
| Alabama and Florida Railroad | L&N | 1850 | 1868 | Pensacola and Louisville Railroad | ||
| Alabama and Florida Railroad | L&N | 1898 | 1900 | Louisville and Nashville Railroad | ||
| Alabama and Florida Railroad | 1936 | 1941 | N/A | |||
| Alabama and Florida Railroad | AFLR | 1986 | 1992 | Alabama and Florida Railway | ||
| Alabama and Florida Railway | AF | 1992 | 2011 | N/A | ||
| Alabama, Florida and Gulf Railroad | 1917 | 1936 | Alabama and Florida Railroad | |||
| Alabama, Florida and Southern Railroad | 1910 | 1917 | Alabama, Florida and Gulf Railroad | |||
| Alabama Great Southern Railroad | AGS | SOU | 1877 | Still exists as an operating subsidiary of the Norfolk Southern Railway | ||
| Alabama Industrial Railroad | AIRI | 1984 | 1985 | Huntsville and Madison County Railroad Authority | ||
| Alabama Midland Railway | ACL | 1887 | 1901 | Savannah, Florida and Western Railway | ||
| Alabama Mineral Railroad | L&N | 1890 | 1903 | Louisville and Nashville Railroad | ||
| Alabama and Mississippi Railroad | A&M | 1902 | 1922 | Mississippi and Alabama Railroad | ||
| Alabama and Mississippi Rivers Railroad | SOU | 1850 | 1864 | Selma and Meridian Railroad | ||
| Alabama Northern Railway | ABN | 1904 | 1925 | |||
| Alabama and North Western Railroad | 1912 | 1934 | N/A | |||
| Alabama Railroad | ALAB | 1991 | 2022 | N/A | ||
| Alabama Southern Railroad | 1985 | 1985 | N/A | Never operated (acquired property of Sumter and Choctaw Railway) | ||
| Alabama, Tennessee and Northern Railroad | SLSF | 1918 | 1971 | St. Louis – San Francisco Railway | ||
| Alabama, Tennessee and Northern Railroad | SLSF | 1906 | 1913 | Alabama, Tennessee and Northern Railway | ||
| Alabama, Tennessee and Northern Railway | SLSF | 1913 | 1918 | Alabama, Tennessee and Northern Railroad | ||
| Alabama and Tennessee River Railroad | SOU | 1848 | 1866 | Selma, Rome and Dalton Railroad | ||
| Alabama Terminal and Improvement Company | ACL | 1889 | 1889 | Alabama Midland Railway | ||
| Alabama Terminal Railroad | ACL | 1907 | 1916 | Atlanta, Birmingham and Atlantic Railway | ||
| Alabama and Tombigbee Railroad | 1898 | N/A | ||||
| Alabama Transit Company | SOU | 1869 | 1870 | Elyton and Aberdeen Railroad | ||
| Alabama Western Railroad | IC | 1905 | 1913 | Chicago, St. Louis and New Orleans Railroad | ||
| Americus, Preston and Lumpkin Railroad | SAL | 1884 | 1888 | Savannah, Americus and Montgomery Railway | ||
| Andalusia and Conecuh Railroad | ACRC | 1983 | Still exists as a lessor of the Three Notch Railroad | |||
| Andalusia, Florida and Gulf Railway | 1919 | 1925 | N/A | |||
| Anniston and Atlantic Railroad | L&N | 1883 | 1890 | Alabama Mineral Railroad | ||
| Anniston and Cincinnati Railroad | L&N | 1887 | 1890 | Alabama Mineral Railroad | ||
| Anniston Terminal Railroad | L&N | 1888 | 1890 | Anniston and Cincinnati Railroad | ||
| Ashland Railway | 1925 | 1940 | N/A | |||
| Atlanta and Birmingham Air Line Railway | SAL | 1903 | 1909 | Seaboard Air Line Railway | ||
| Atlanta, Birmingham and Atlantic Railroad | ACL | 1905 | 1916 | Atlanta, Birmingham and Atlantic Railway | ||
| Atlanta, Birmingham and Atlantic Railway | ACL | 1916 | 1927 | Atlanta, Birmingham and Coast Railroad | ||
| Atlanta, Birmingham and Coast Railroad | ACL | 1927 | 1946 | Atlantic Coast Line Railroad | ||
| Atlanta and St. Andrews Bay Railway | A&SA, ASAB | 1906 | 1993 | Bay Line Railroad | ||
| Atlantic Coast Line Railroad | ACL | ACL | 1902 | 1967 | Seaboard Coast Line Railroad | |
| Bay Minette and Fort Morgan Railroad | L&N | 1904 | 1921 | Louisville and Nashville Railroad | ||
| Belt Line Railway | ACL | 1889 | 1943 | Atlantic Coast Line Railroad | ||
| Birmingham and Atlanta Air Line Railway | SAL | 1902 | 1903 | East and West Railroad | ||
| Birmingham and Atlantic Railroad | 1890 | 1919 | N/A | |||
| Birmingham Belt Railroad | SLSF | 1899 | 1968 | St. Louis – San Francisco Railway | ||
| Birmingham Mineral Railroad | L&N | 1884 | 1904 | Louisville and Nashville Railroad | ||
| Birmingham, Selma and Mobile Railroad | 1913 | 1931 | N/A | |||
| Birmingham, Selma and New Orleans Railway | L&N | 1886 | 1902 | Louisville and Nashville Railroad | ||
| Birmingham, Selma and Pensacola Railroad | 1910 | 1913 | Birmingham, Selma and Mobile Railroad | |||
| Birmingham, Sheffield and Tennessee River Railway | SOU | 1889 | 1895 | Northern Alabama Railway | ||
| Birmingham and Southeastern Railway | B&SE | 1911 | 1965 | N/A | ||
| Birmingham and Tennessee River Railroad | SOU | 1884 | 1885 | Sheffield and Birmingham Railroad | ||
| Birmingham Southern Railroad | BS | 1899 | 2012 | Birmingham Terminal Railway | ||
| Birmingham Terminal Company | BTC | CG/ IC/ SAL/ SLSF/ SOU | ||||
| Birmingham and Tuscaloosa Railroad | L&N | 1915 | 1915 | Louisville and Nashville Railroad | ||
| Brierfield, Blockton and Birmingham Railway | SOU | 1889 | 1889 | East Tennessee, Virginia and Georgia Railway | ||
| Burlington Northern Inc. | BN | 1980 | 1981 | Burlington Northern Railroad | ||
| Burlington Northern Railroad | BN | 1981 | 1996 | Burlington Northern and Santa Fe Railway | ||
| Cahaba, Marion and Greensboro Railroad | SOU | 1860 | 1868 | Selma, Marion and Memphis Railroad | ||
| Canton, Aberdeen and Nashville Railroad in Alabama | IC | 1898 | 1913 | Chicago, St. Louis and New Orleans Railroad | ||
| Carrollton Short Line Railway | SLSF | 1897 | 1906 | Alabama, Tennessee and Northern Railroad | ||
| Central of Georgia Railway | CG | CG | 1895 | 1971 | Central of Georgia Railroad | |
| Chattahoochee and Gulf Railroad | CHAT | 2003 | 2006 | Chattahoochee Bay Railroad | ||
| Chattahoochee and Gulf Railroad | CG | 1899 | 1948 | Central of Georgia Railway | ||
| Chattahoochee Valley Railroad | 1895 | 1900 | Chattahoochee Valley Railway | |||
| Chattahoochee Valley Railway | CHV | 1900 | 1992 | N/A | ||
| Chattanooga Southern Railroad | SOU | 1896 | 1911 | Tennessee, Alabama and Georgia Railroad | ||
| Chattanooga Southern Railway | SOU | 1890 | 1895 | Chattanooga Southern Railroad | ||
| Cheney Railroad | CHNY | 1989 | 1996 | N/A | ||
| Chicago, St. Louis and New Orleans Railroad | IC | 1913 | 1951 | Illinois Central Railroad | ||
| Cincinnati, Selma and Mobile Railway | SOU | 1881 | 1890 | East Tennessee, Virginia and Georgia Railway | ||
| Columbus, Fayette and Decatur Railroad | SOU | 1871 | 1881 | Georgia Pacific Railway | ||
| Columbus and Western Railway | CG | 1880 | 1888 | Savannah and Western Railroad | ||
| Decatur, Chesapeake and New Orleans Railway | L&N | 1887 | 1893 | Middle Tennessee and Alabama Railway | ||
| DeKalb and Western Railroad | 1916 | N/A | ||||
| East Alabama Railway | CG | 1880 | 1888 | Savannah and Western Railroad | ||
| East Alabama and Cincinnati Railroad | CG | 1868 | 1880 | East Alabama and Cincinnati Railway | ||
| East Alabama and Cincinnati Railway | CG | 1880 | 1880 | East Alabama Railway | ||
| East Tennessee, Virginia and Georgia Railroad | SOU | 1881 | 1886 | East Tennessee, Virginia and Georgia Railway | ||
| East Tennessee, Virginia and Georgia Railway | SOU | 1886 | 1894 | Southern Railway | ||
| East and West Railroad | SAL | 1894 | 1903 | Atlanta and Birmingham Air Line Railway | ||
| East and West Railroad of Alabama | SAL | 1882 | 1893 | East and West Railroad | ||
| Eastern Railway of Alabama | ACL | 1901 | 1906 | Atlanta, Birmingham and Atlantic Railroad | ||
| Elyton and Aberdeen Railroad | SOU | 1870 | 1882 | Georgia Pacific Railway | ||
| Ensley Southern Railway | SOU | 1900 | 1926 | Southern Railway, Warrior River Terminal Company | ||
| Escambia Railroad | ||||||
| Eufaula and East Alabama Railway | CG | 1887 | 1888 | Savannah and Western Railroad | ||
| Eufaula and Clayton Railway | CG | 1883 | 1888 | Savannah and Western Railroad | ||
| Florence Bridge Company | SOU | 1832 | 1850 | Memphis and Charleston Railroad | ||
| Florence Railroad and Improvement Company | L&N | 1888 | 1897 | Louisville and Nashville Railroad | ||
| Florida and Alabama Railroad | ||||||
| Florida, Alabama and Gulf Railroad | 1911 | 1919 | Andalusia, Florida and Gulf Railway | |||
| Gadsden and Attalla Railroad | SOU | 1876 | 1904 | Southern Railway | ||
| Gadsden and Birmingham Railroad | SOU | 1910 | 1911 | Tennessee, Alabama and Georgia Railroad | ||
| Gainesville and Mississippi Railroad | GM&O | 1852 | 1854 | Mississippi, Gainesville and Tuscaloosa Railroad | ||
| Georgia and Alabama Railroad | GAAB | 1989 | 1995 | Georgia Southwestern Railroad | ||
| Georgia and Alabama Railway | SAL | 1895 | 1902 | Seaboard Air Line Railway | ||
| Georgia Pacific Railroad | SOU | 1876 | 1882 | Georgia Pacific Railway | ||
| Georgia Pacific Railway | SOU | 1882 | 1894 | Southern Railway | ||
| Georgia Western Railroad | SOU | 1860 | 1877 | Georgia Pacific Railroad | ||
| Girard Railroad | CG | 1845 | 1854 | Mobile and Girard Railroad | ||
| Goodwater and Birmingham Railroad | CG | 1886 | 1887 | Columbus and Western Railway | ||
| Gulf, Florida and Alabama Railway | DWR | SLSF | 1911 | 1922 | Muscle Shoals, Birmingham and Pensacola Railway | |
| Gulf and Mississippi Railroad | GMSR | 1985 | 1988 | SouthRail Corporation | ||
| Gulf, Mobile and Northern Railroad | GM&N | GM&O | 1915 | 1940 | Gulf, Mobile and Ohio Railroad | |
| Gulf, Mobile and Ohio Railroad | GM&O, GMO | GM&O | 1940 | 1972 | Illinois Central Gulf Railroad | |
| Gulf Ports Terminal Railway | 1916 | 1926 | N/A | |||
| Gulf Terminal Company | GM&O/ SOU | |||||
| Hartford and Slocomb Railroad | HS | 1953 | 1992 | H&S Railroad | ||
| Hayneville and Montgomery Railroad | 1905 | 1917 | [2] | |||
| H&S Railroad | HS | 1992 | 2006 | Chattahoochee Bay Railroad | ||
| Huntsville and Elora Railroad | L&N | 1887 | 1887 | Nashville, Chattanooga and St. Louis Railway | ||
| Illinois Central Railroad | IC | IC | 1899 | 1972 | Illinois Central Gulf Railroad | |
| Illinois Central Gulf Railroad | ICG | 1972 | 1988 | Illinois Central Railroad | ||
| Indiana, Alabama and Texas Railroad | L&N | 1881 | 1887 | Louisville and Nashville Railroad | ||
| Inland Waterways Corporation | 1948 | 1966 | Birmingham Southern Railroad | |||
| Jefferson Warrior Railroad | 1985 | 2009 | Alabama Warrior Railway | |||
| Kansas City, Memphis and Birmingham Railroad | SLSF | 1887 | 1928 | St. Louis – San Francisco Railway | ||
| Kansas City Southern Railway | KCS | 1994 | 2005 | Alabama Southern Railroad | ||
| Long Branch Coal Railroad | L&N | 1901 | 1957 | Louisville and Nashville Railroad | ||
| Louisville and Nashville Railroad | L&N, LN | L&N | 1871 | 1983 | Seaboard System Railroad | |
| Manistee and Repton Railroad | L&N | 1910 | 1965 | Louisville and Nashville Railroad | ||
| Marion Railroad | SOU | 1854 | 1858 | Marion and Cahaba Railroad | ||
| Marion and Alabama River Transportation Company | SOU | 1850 | 1854 | Marion Railroad | ||
| Marion and Cahaba Railroad | SOU | 1858 | 1860 | Cahaba, Marion and Greensboro Railroad | ||
| Mary Lee Railroad | 1985 | Jefferson Warrior Railroad | private | |||
| Meridian and Bigbee Railroad | MBRR, MB | 1952 | 1997 | M&B Railroad | ||
| Meridian and Bigbee River Railway | 1917 | 1952 | Meridian and Bigbee Railroad | |||
| Memphis and Birmingham Railroad | SLSF | 1886 | 1887 | Kansas City, Memphis and Birmingham Railroad | ||
| Memphis and Charleston Railroad | SOU | 1850 | 1898 | Southern Railway | ||
| Middle Tennessee and Alabama Railway | L&N | 1893 | 1897 | Nashville, Chattanooga and St. Louis Railway | ||
| Mississippi and Alabama Railroad | 1922 | 1950 | N/A | |||
| Mississippi Central Railroad | MSC | IC | 1921 | 1924 | N/A | Trackage rights over the Gulf, Mobile and Northern Railroad to Mobile |
| Mississippi, Gainesville and Tuscaloosa Railroad | GM&O | 1854 | 1870 | Mobile and Ohio Railroad | ||
| Mobile and Alabama Grand Trunk Railroad | SOU | 1866 | 1886 | Mobile and West Alabama Railroad | ||
| Mobile and Bay Shore Railway | GM&O | 1898 | 1903 | Mobile and Ohio Railroad | ||
| Mobile and Birmingham Railroad | SOU | 1895 | Still exists as a nonoperating subsidiary of the Norfolk Southern Railway | |||
| Mobile and Birmingham Railway | SOU | 1887 | 1895 | Mobile and Birmingham Railroad | ||
| Mobile and Girard Railroad | CG | 1854 | 1895 | Central of Georgia Railway | ||
| Mobile and Great Northern Railroad | L&N | 1856 | 1868 | Mobile and Montgomery Railroad | ||
| Mobile and Gulf Railroad | MG | 1925 | 1984 | N/A[3] | ||
| Mobile, Hattiesburg and Jackson Railroad | GM&O | 1887 | 1888 | Mobile, Jackson and Kansas City Railroad | ||
| Mobile, Jackson and Kansas City Railroad | GM&O | 1888 | 1909 | New Orleans, Mobile and Chicago Railroad | ||
| Mobile and Montgomery Railroad | L&N | 1868 | 1874 | Mobile and Montgomery Railway | ||
| Mobile and Montgomery Railway | L&N | 1874 | 1900 | Louisville and Nashville Railroad | ||
| Mobile and Northwestern Railroad | GM&O | 1870 | 1891 | Mobile, Jackson and Kansas City Railroad | ||
| Mobile and Ohio Railroad | GM&O | 1848 | 1940 | Gulf, Mobile and Ohio Railroad | ||
| Mobile Terminal and Railway Company | SLSF | 1910 | 1913 | Alabama, Tennessee and Northern Railway | ||
| Mobile and West Alabama Railroad | SOU | 1885 | 1887 | Mobile and Birmingham Railway | ||
| Mobile and Western Railroad | GM&O | 1906 | 1907 | Mobile and Ohio Railroad | ||
| Montgomery Railroad | ACL/ L&N | 1834 | 1842 | Montgomery and West Point Railroad | ||
| Montgomery and Eufaula Railroad | CG | 1860 | 1879 | Montgomery and Eufaula Railway | ||
| Montgomery and Eufaula Railway | CG | 1879 | 1895 | Central of Georgia Railway | ||
| Montgomery and Florida Railway | ACL | 1886 | 1888 | Northwestern and Florida Railroad | ||
| Montgomery, Hayneville and Camden Railroad | L&N | 1890 | 1900 | Louisville and Nashville Railroad | ||
| Montgomery and Prattville Railroad | L&N | 1895 | 1896 | Louisville and Nashville Railroad | ||
| Montgomery Southern Railway | ACL | 1880 | 1886 | Montgomery and Florida Railway | ||
| Montgomery and West Point Railroad | ACL/ L&N | 1843 | 1870 | Western Railroad of Alabama | ||
| Muscle Shoals, Birmingham and Pensacola Railroad | MSBP | SLSF | 1924 | 1928 | St. Louis – San Francisco Railway | |
| Muscle Shoals, Birmingham and Pensacola Railway | MSBP | SLSF | 1922 | 1924 | Muscle Shoals, Birmingham and Pensacola Railroad | |
| Nashville and Chattanooga Railroad | L&N | 1850 | 1873 | Nashville, Chattanooga and St. Louis Railway | ||
| Nashville, Chattanooga and St. Louis Railway | N&C, NC | L&N | 1873 | 1957 | Louisville and Nashville Railroad | |
| Nashville and Decatur Railroad | L&N | 1866 | 1990 | CSX Transportation | ||
| Nashville, Florence and Sheffield Railway | L&N | 1887 | 1900 | Louisville and Nashville Railroad | ||
| New and Old Decatur Belt and Terminal Railroad | L&N | 1890 | 1892 | Louisville and Nashville Railroad | ||
| New Orleans, Mobile and Chattanooga Railroad | L&N | 1866 | 1871 | New Orleans, Mobile and Texas Railroad | ||
| New Orleans, Mobile and Chicago Railroad | GM&O | 1909 | 1915 | Gulf, Mobile and Northern Railroad | ||
| New Orleans, Mobile and Texas Railroad | L&N | 1871 | 1881 | Louisville and Nashville Railroad | ||
| New Orleans and Selma Railroad | L&N | 1866 | 1868 | New Orleans and Selma Railroad and Immigration Association | ||
| New Orleans and Selma Railroad and Immigration Association | L&N | 1868 | 1886 | Birmingham, Selma and New Orleans Railway | ||
| North Alabama Railroad | L&N | 1900 | 1910 | Louisville and Nashville Railroad | ||
| North East and South West Alabama Railroad | SOU | 1853 | 1868 | Alabama and Chattanooga Railroad | ||
| Northern Alabama Railway | SOU | 1895 | 1939 | Southern Railway | ||
| Northwestern Railroad of Alabama | SOU | 1854 | 1868 | Selma, Marion and Memphis Railroad | ||
| Northwestern and Florida Railroad | ACL | 1888 | 1889 | Alabama Terminal and Improvement Company | ||
| Oak Grove and Georgetown Railroad | 1904 | 1927 | N/A[4] | |||
| Oneonta and Attalla Railroad | L&N | 1900 | 1905 | Louisville and Nashville Railroad | ||
| Opelika and Talladega Railroad | CG | 1854 | 1861 | Opelika and Tuscumbia Railroad | ||
| Opelika and Tuscumbia Railroad | CG | 1861 | 1866 | Savannah and Memphis Railroad | ||
| Pensacola, Mobile and New Orleans Railway | 1907 | 1917 | Gulf Ports Terminal Railway | |||
| Pensacola and Selma Railroad | L&N | 1880 | 1880 | Louisville and Nashville Railroad | ||
| Pine Belt Southern Railroad | PBRR | 1995 | 2001 | N/A | ||
| Plant System | ACL | 1894 | 1902 | Atlantic Coast Line Railroad | ||
| Richmond and Danville Railroad | SOU | 1889 | 1894 | Southern Railway | ||
| Rome and Decatur Railroad | SOU | 1882 | 1889 | East Tennessee, Virginia and Georgia Railway | ||
| St. Louis and San Francisco Railroad | SLSF | 1903 | 1916 | St. Louis – San Francisco Railway | ||
| St. Louis – San Francisco Railway | SLSF | SLSF | 1916 | 1980 | Burlington Northern Inc. | |
| Savannah, Americus and Montgomery Railway | SAL | 1888 | 1895 | Georgia and Alabama Railway | ||
| Savannah, Florida and Western Railway | ACL | 1901 | 1902 | Atlantic Coast Line Railroad | ||
| Savannah and Memphis Railroad | CG | 1866 | 1880 | Columbus and Western Railway | ||
| Savannah and Western Railroad | CG | 1885 | 1895 | Central of Georgia Railway | ||
| Seaboard Railroad of Alabama | 1890 | 1900 | Tombigbee and Northern Railway | |||
| Seaboard Air Line Railroad | SAL | SAL | 1946 | 1967 | Seaboard Coast Line Railroad | |
| Seaboard Air Line Railway | SAL | 1900 | 1945 | Seaboard Air Line Railroad | ||
| Seaboard Coast Line Railroad | SCL | 1967 | 1983 | Seaboard System Railroad | ||
| Seaboard System Railroad | SBD | 1983 | 1986 | CSX Transportation | ||
| Selma and Gulf Railroad | L&N | 1858 | 1879 | Pensacola and Selma Railroad | ||
| Selma and Greensboro Railroad | SOU | 1878 | 1881 | Cincinnati, Selma and Mobile Railway | ||
| Selma, Marion and Memphis Railroad | SOU | 1868 | 1878 | Selma and Greensboro Railroad | ||
| Selma and Meridian Railroad | SOU | 1864 | 1871 | Alabama Central Railroad | ||
| Selma, Rome and Dalton Railroad | SOU | 1866 | 1880 | East Tennessee, Virginia and Georgia Railroad | ||
| Sheffield and Birmingham Railroad | SOU | 1885 | 1887 | Sheffield and Birmingham Coal, Iron and Railway Company | ||
| Sheffield and Birmingham Coal, Iron and Railway Company | SOU | 1887 | 1889 | Birmingham, Sheffield and Tennessee River Railway | ||
| Sheffield and Tuscumbia Railroad | L&N | 1895 | 1896 | Louisville and Nashville Railroad | ||
| Sheffield and Tuscumbia Street Railway | L&N | 1886 | 1895 | Sheffield and Tuscumbia Railroad | ||
| South and North Alabama Railroad | L&N | 1854 | 1914 | Louisville and Nashville Railroad | ||
| Southern Railway | SOU | SOU | 1894 | 1990 | Norfolk Southern Railway | |
| Southern Alabama Railroad | SUAB | 1988 | 2001 | Conecuh Valley Railroad | ||
| Southern Alabama Railroad | L&N | 1899 | 1899 | Louisville and Nashville Railroad | ||
| SouthRail Corporation | SR | 1988 | 1994 | Kansas City Southern Railway | ||
| Southwestern Railroad | CG | 1859 | Owned by Central of Georgia Railway; still exists as a lessor of Norfolk Southern Railway operating subsidiary Central of Georgia Railroad | |||
| Southwestern Alabama Railway | ACL | 1896 | 1901 | Savannah, Florida and Western Railway | ||
| Sumter and Choctaw Railway | SC | 1904 | 1983 | Alabama Southern Railroad (never operated) | ||
| Sylacauga and Wetumpka Railroad | 1902 | 1911 | [5] | |||
| Talladega and Coosa Valley Railroad | 1883 | 1890 | Birmingham and Atlantic Railroad | |||
| Tallassee and Montgomery Railway | 1895 | 1912 | Birmingham and Southeastern Railway | |||
| Tennessee and Alabama Railroad | L&N | 1887 | 1887 | Nashville, Florence and Sheffield Railway | ||
| Tennessee and Alabama Central Railroad | L&N | 1853 | 1866 | Nashville and Decatur Railroad | ||
| Tennessee, Alabama and Georgia Railroad | SOU | 1911 | 1922 | Tennessee, Alabama and Georgia Railway | ||
| Tennessee, Alabama and Georgia Railway | TA&G, TAG | SOU | 1922 | Still exists as an operating subsidiary of the Norfolk Southern Railway | ||
| Tennessee Coal and Iron Railroad | ||||||
| Tennessee and Coosa Railroad | L&N | 1844 | 1891 | Nashville, Chattanooga and St. Louis Railway | ||
| Tennessee Valley Railroad | SOU | 1848 | 1851 | Memphis and Charleston Railroad | ||
| Tombigbee and Northern Railway | 1900 | 1904 | Tombigbee Valley Railroad | |||
| Tombigbee Valley Railroad | SLSF | 1904 | 1913 | Alabama, Tennessee and Northern Railway | ||
| Tredegar Mineral Railway | SAL | 1891 | 1900 | East and West Railroad | ||
| Tuscaloosa Mineral Railroad | L&N | 1910 | 1915 | Birmingham and Tuscaloosa Railroad | ||
| Tuscumbia Railway | SOU | 1830 | 1850 | Memphis and Charleston Railroad | ||
| Tuscumbia, Courtland and Decatur Railroad | SOU | 1832 | 1847 | Tennessee Valley Railroad | ||
| Tuskegee Railroad | TK | 1860 | 1963 | N/A | ||
| Tyson Railroad | TSNR | 1989 | 1992 | CSX Transportation | ||
| Union Springs and Northern Railway | 1901 | 1911 | Birmingham and Southeastern Railway | |||
| Vicksburg and Brunswick Railroad | CG | 1867 | 1879 | Eufaula and Clayton Railway | ||
| Vredenburgh Saw Mill Company | ||||||
| Warrior River Terminal Company | 1926 | 1948 | Inland Waterways Corporation | |||
| Warrior Southern Railway | 1901 | 1938 | Twin Seam Mining Company | Owned by Mobile and Ohio Railroad | ||
| Washington and Choctaw Railway | 1910 | 1927 | N/A | |||
| Western Railroad of Alabama | ACL/ L&N | 1860 | 1883 | Western Railway of Alabama | ||
| Western Railway of Alabama | WA | ACL/ L&N | 1883 | 2002 | CSX Transportation | |
| Wills Valley Railroad | SOU | 1852 | 1868 | Alabama and Chattanooga Railroad | ||
| Winchester and Alabama Railroad | SOU | 1850 | 1875 | Memphis and Charleston Railroad | ||
| Woodstock and Blocton Railway | L&N/ SOU | 1906 | Still exists; owned by CSX Transportation and the Norfolk Southern Railway but operated by the latter | |||
| Woodward Iron Company | private | |||||
| Yellow River Railroad | L&N | 1887 | 1906 | Louisville and Nashville Railroad |