Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase explained

Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase
Ec Number:2.4.99.12

Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (KDO transferase, waaA (gene), kdtA (gene), 3-deoxy-D-manno-oct-2-ulosonic acid transferase, 3-deoxy-manno-octulosonic acid transferase, lipid IVA KDO transferase) is an enzyme with systematic name CMP-3-deoxy-D-manno-oct-2-ulosonate:lipid IVA 3-deoxy-D-manno-oct-2-ulosonate transferase.[1] [2] [3] [4] This enzyme catalyses the following chemical reaction

lipid IVA + CMP-alpha-Kdo

\rightleftharpoons

alpha-Kdo-(2->6)-lipid IVA + CMP

The bifunctional enzyme from Escherichia coli transfers two 3-deoxy-D-man no-oct-2-resonate residues to lipid IVA.

See also

Notes and References

  1. Belunis CJ, Raetz CR . Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli . The Journal of Biological Chemistry . 267 . 14 . 9988–97 . May 1992 . 10.1016/S0021-9258(19)50189-2 . free . 1577828 .
  2. Mamat U, Schmidt H, Munoz E, Lindner B, Fukase K, Hanuszkiewicz A, Wu J, Meredith TC, Woodard RW, Hilgenfeld R, Mesters JR, Holst O . WaaA of the hyperthermophilic bacterium Aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis . The Journal of Biological Chemistry . 284 . 33 . 22248–62 . August 2009 . 19546212 . 2755949 . 10.1074/jbc.M109.033308 . free .
  3. White KA, Kaltashov IA, Cotter RJ, Raetz CR . A mono-functional 3-deoxy-D-manno-octulosonic acid (Kdo) transferase and a Kdo kinase in extracts of Haemophilus influenzae . The Journal of Biological Chemistry . 272 . 26 . 16555–63 . June 1997 . 9195966 . 10.1074/jbc.272.26.16555 . free .
  4. Löbau S, Mamat U, Brabetz W, Brade H . Molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdtA encoding 3-deoxy-alpha-D-man no-octulosonic acid transferase of Chlamydia pneumoniae strain TW-183 . Molecular Microbiology . 18 . 3 . 391–9 . November 1995 . 8748024 . 10.1111/j.1365-2958.1995.mmi_18030391.x .