| Winner: | Alyssa Louise Erika Tia Jimenea |
| Represented: | Victorias City |
| Date: | April 18, 2016 |
| Presenters: | Benjamin Alves, Max Collins |
| Venue: | Pana-ad Stadium, Bacolod, Negros Occidental, Philippines |
| Entrants: | 23 |
| Returns: | Bacolod, Ilog, Pontevedra, Sipalay City |
Lin-ay sang Negros 2016, the 22nd edition of the annual Lin-ay sang Negros pageant was held on April 18, 2016 at the Pana-ad Stadium.[1] A total of 11 cities and 12 municipalities sent their representatives. Lin-ay sang Negros 2015 winner Jessica Zevenbergen of San Carlos City, crowned her successor Alyssa Jimenea of Victorias at the end of the event.[2] This is the first time the pageant is held with the province no longer a part of Western Visayas but the Negros Island Region.[3]
| Final Result | Contestant | |
|---|---|---|
| Lin-ay sang Negros 2016 | Victorias - Alyssa Jimenea | |
| 1st Runner-Up | Murcia - Louise Lian Enumerable | |
| 2nd Runner Up | Sipalay - Artmarie Getonzo | |
| Top 5 |
| |
| Top 10 |
|
| Contestant | Name | |
|---|---|---|
| Bacolod | Ivy Benedicto | |
| Bago City | Justine Marie Baer | |
| Binalbagan | Cindy Angelou Yanga | |
| Cadiz | Ernalyn Aureta | |
| Calatrava | Sarah Magne Bautista | |
| Candoni | Allen Gigante | |
| Cauayan | Princess Dahlia Gulucson | |
| E.B. Magalona | Johan Marie Javelosa | |
| Hinigaran | Danielle Rose Ferraren | |
| Hinoba-an | Marianne Dalipe | |
| Ilog | Maria Jamela Fortaleza | |
| Vanessa Keith Adera | ||
| La Carlota City | Juna Mae Perez | |
| Murcia | Louise Lian Enumerable | |
| Pontevedra | Hezamae Gerale | |
| Sagay City | Paolyn Louise Lacson | |
| Don Salvador Benedicto | Jemmel Badera | |
| Roselle Ann Ramas | ||
| Erna Torreblancea | ||
| Sipalay City | Artmarie Getonzo | |
| Talisay City | Febbie Merivelez | |
| Toboso | Prairie Mandajoyan | |
| Alyssa Jimenea |