Lillie's trichrome explained

Lillie's trichrome is a combination of dyes used in histology.

It is similar to Masson's trichrome stain, but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940.[1] It is applied by submerging the fixated sample into the following three solutions:[2] Weigert's iron hematoxylin working solution, Biebrich scarlet solution, and Fast Green FCF solution.

The resulting stains are black cell nuclei, brown cytoplasm, red muscle and myelinated fibers, blue collagen, and scarlet erythrocytes.[3]

Applications

Trichrome stains are normally used to differentiate between collagen and muscle tissues. Some studies that benefit from its application include end stage liver disease (cirrhosis), myocardial infarction, muscular dystrophy, and tumor analysis.[4]

External links

Notes and References

  1. Lillie . R. D. . 1940 . Further Experiments with the Masson Trichrome Modification of Mallory's Connective Tissue Stain . . 15 . 1 . 17–22 . 10.3109/10520294009110327.
  2. Tchertkoff. Victor. 1962-10-01. Staining Procedures Used by the Biological Stain Commission, ed. 2. Revised by H. J. Conn, et al. Baltimore, Md., The Williams & Wilkins Co., 1960. $5.00. Clinical Chemistry. 8. 5. 548. 10.1093/clinchem/8.5.548a. 0009-9147. free.
  3. LILLIE . WALTER I. . Prechiasmal Syndrome Produced by Chronic Local Arachnoiditis . 1940-11-01 . . 24 . 5 . 940 . 10.1001/archopht.1940.00870050090006 . 0093-0326.
  4. Web site: Cowie . Robert . Masson's Trichrome Stain . Science Direct.