| Leslie Barnett | |
| Birth Date: | 12 October 1920 |
| Birth Place: | London, England |
| Other Names: | Margaret Leslie Collard |
| Known For: | Frameshift mutations |
| Occupation: | Molecular biologist |
Leslie Barnett (born 12 October 1920 as Margaret Leslie Collard – died 10 February 2002)[1] was a British biologist who worked with Francis Crick, Sydney Brenner, and Richard J. Watts-Tobin to genetically demonstrate the triplet nature of the code of protein translation through the Crick, Brenner, Barnett, Watts-Tobin et al. experiment of 1961, which discovered frameshift mutations; this insight provided early elucidation of the nature of the genetic code.
In her professional life, Barnett was a microbiologist who joined the Laboratory of Molecular Biology in Cambridge, where she worked as an assistant mainly with Crick and later Brenner, with whom she remained for many years until he left and she retired. During this long period, Barnett was involved in a number of the important 20th-century advances made in molecular biology and genetics.[2]
At the beginning of the Second World War, she had started training in the Institute of Agriculture in Essex; but in 1939 took a job in Felixstowe as a milk testing apprentice at a local dairy, and later with United Dairies in London. After evacuation to Banbury and several further jobs she returned to London where she met and married James in 1945. He insisted that she took up the offer of a place at Reading University and she remembered these as some of the happiest days of her life, studying for her BSc in dairying. Leslie continued to be fond of James despite their divorce when the children were quite young. She had two daughters, Penny and Marion.
Barnett came to work as a technician in the MRC Unit[3] shortly before the move from the Cavendish Laboratory to "The Hut" in Cambridge, which would later go on to become the MRC Laboratory of Molecular Biology (LMB). Her role was to help with the computing for the crystallographers. On the arrival of Sydney Brenner, however, help was needed to set up the phage research and then to prepare for the arrival of Seymour Benzer and other American visitors that autumn. Leslie worked with Vernon Ingram in determining the GLU to VAL amino acid change in the beta chain of Hb responsible for the sickle cell phenotype; this was the first molecular disease characterized. Barnett showed her versatility when she transferred to Brenner's programme and made a major contribution to the laboratory work, becoming a co-author of their two major papers on the results. In 1966 she was appointed Senior Tutor at the new graduate college, Clare Hall, Cambridge. She proved very popular in this work.
On Brenner's retirement in 1986, Barnett left the LMB to work for him in the new MRC Molecular Genetics Unit in Addenbrooke's Hospital. She trained the late Francis Crick in experimental work on phage and assisted him.
Barnett also helped set up Sydney Brenner's laboratory in Singapore, many years later.
Barnett was amongst the early Fellows elected to join Clare Hall, the newly founded graduate college. In 1972, she became a tutor for graduate students; in 1975, she took over the leading role of Senior Tutor, which she held until her retirement in 1985. She was remembered by hundreds of students of various nationalities, ages and subjects for her "steadfast care, wisdom in solving their problems, and motherly love."
Barnett played a leading role in college affairs, particularly in the organising of social events and parties and will be remembered with great affection by her colleagues and other friends associated with the college. As a token of her contribution to the college the Governing Body has named a student residence the Leslie Barnett House, and a bronze head by the late Lois Tilbrook represents another tribute to her on the site. On retirement she was elected to an Emeritus Fellowship and attended regularly until her last illness.
Eric Miller, who was a visiting scientist at the Molecular Biology Laboratory in 1986–87, wrote: "Leslie Barnett should be recognised for her immense scientific contributions to the field of gene structure and function. Professionally, to which I am sure Francis Crick and Sydney Brenner will attest, many of the seminal discoveries in molecular biology would not have occurred without the careful hand, watchful eye and steadfast focus that Leslie brought to the bench and experiments." Francis Crick said: "Alex Rich and I engaged Leslie in 1955 to do some x-ray calculations for us, since in those days electronic computers were still very primitive. After we discovered that Leslie had biological qualifications she did various types of experimental work. Among other things she worked with us on the phase-shift mutants of phage T4. She was invaluable, as she was always such a careful and meticulous experimentalist."
In 1978 at Clare Hall a second neighbouring house in Cambridge, now called Leslie Barnett House, was obtained for graduate student accommodation; it was named after the popular Senior Tutor.