Lens fiber major intrinsic protein explained

Lens fiber major intrinsic protein also known as aquaporin-0 is a protein that in humans is encoded by the MIP gene.^{[1] [2] [3]}

Function

Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13.^[3]

Further reading

• Chepelinsky AB, Piatigorsky J, Pisano MM, etal . Lens protein gene expression: alpha-crystallins and MIP. . Lens and Eye Toxicity Research . 8 . 2–3 . 319–44 . 1991 . 1911643 .
• Griffin CS, Shiels A . In situ hybridisation localises the gene for the major intrinsic protein of eye lens fibre cell membranes to human chromosome 12q14 . Cytogenet. Cell Genet. . 61 . 1 . 8–9 . 1992 . 1505237 . 10.1159/000133360 .
• Girsch SJ, Peracchia C . Calmodulin interacts with a C-terminus peptide from the lens membrane protein MIP26 . Curr. Eye Res. . 10 . 9 . 839–49 . 1992 . 1790714 . 10.3109/02713689109013880 .
• Lampe PD, Johnson RG . Amino acid sequence of in vivo phosphorylation sites in the main intrinsic protein (MIP) of lens membranes . Eur. J. Biochem. . 194 . 2 . 541–7 . 1991 . 2176601 . 10.1111/j.1432-1033.1990.tb15650.x . free .
• Manenti S, Dunia I, Benedetti EL . Fatty acid acylation of lens fiber plasma membrane proteins. MP26 and alpha-crystallin are palmitoylated . FEBS Lett. . 262 . 2 . 356–8 . 1990 . 2335219 . 10.1016/0014-5793(90)80228-B . 40684631 . free .
• Peracchia C, Girsch SJ . Calmodulin site at the C-terminus of the putative lens gap junction protein MIP26 . Lens and Eye Toxicity Research . 6 . 4 . 613–21 . 1991 . 2487274 .
• Saito F, Sasaki S, Chepelinsky AB, etal . Human AQP2 and MIP genes, two members of the MIP family, map within chromosome band 12q13 on the basis of two-color FISH . Cytogenet. Cell Genet. . 68 . 1–2 . 45–8 . 1994 . 7525161 . 10.1159/000133885 .
• Jarvis LJ, Louis CF . Purification and oligomeric state of the major lens fiber cell membrane proteins . Curr. Eye Res. . 14 . 9 . 799–808 . 1996 . 8529419 . 10.3109/02713689508995802 .
• Wang XY, Ohtaka-Maruyama C, Pisano MM, etal . Isolation and characterization of the 5'-flanking sequence of the human ocular lens MIP gene . Gene . 167 . 1–2 . 321–5 . 1996 . 8566800 . 10.1016/0378-1119(95)00637-0 .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Prabhakaram M, Katz ML, Ortwerth BJ . Glycation mediated crosslinking between alpha-crystallin and MP26 in intact lens membranes . Mech. Ageing Dev. . 91 . 1 . 65–78 . 1997 . 8910261 . 10.1016/0047-6374(96)01781-2 . 53227438 .
• Ma T, Yang B, Umenishi F, Verkman AS . Closely spaced tandem arrangement of AQP2, AQP5, and AQP6 genes in a 27-kilobase segment at chromosome locus 12q13 . Genomics . 43 . 3 . 387–9 . 1997 . 9268644 . 10.1006/geno.1997.4836 .
• Ohtaka-Maruyama C, Wang X, Ge H, Chepelinsky AB . Overlapping Sp1 and AP2 binding sites in a promoter element of the lens-specific MIP gene . Nucleic Acids Res. . 26 . 2 . 407–14 . 1998 . 9421492 . 10.1093/nar/26.2.407 . 147274 .
• Dunia I, Recouvreur M, Nicolas P, etal . Assembly of connexins and MP26 in lens fiber plasma membranes studied by SDS-fracture immunolabeling . J. Cell Sci. . 111 . 15. 2109–20 . 1998 . 10.1242/jcs.111.15.2109 . 9664032 . free .
• Schey KL, Fowler JG, Shearer TR, David L . Modifications to rat lens major intrinsic protein in selenite-induced cataract . Invest. Ophthalmol. Vis. Sci. . 40 . 3 . 657–67 . 1999 . 10067969 .
• Schey KL, Little M, Fowler JG, Crouch RK . Characterization of human lens major intrinsic protein structure . Invest. Ophthalmol. Vis. Sci. . 41 . 1 . 175–82 . 2000 . 10634618 .
• Berry V, Francis P, Kaushal S, etal . Missense mutations in MIP underlie autosomal dominant 'polymorphic' and lamellar cataracts linked to 12q . Nat. Genet. . 25 . 1 . 15–7 . 2000 . 10802646 . 10.1038/75538 . 22878198 .

Notes and References

1. Pisano MM, Chepelinsky AB . Genomic cloning, complete nucleotide sequence, and structure of the human gene encoding the major intrinsic protein (MIP) of the lens . Genomics . 11 . 4 . 981–90 . Mar 1992 . 1840563 . 10.1016/0888-7543(91)90023-8 .
2. Mulders SM, Preston GM, Deen PM, Guggino WB, van Os CH, Agre P . Water channel properties of major intrinsic protein of lens . J Biol Chem . 270 . 15 . 9010–16 . May 1995 . 7536742 . 10.1074/jbc.270.15.9010 . 2066/21519 . free . free .
3. Web site: Entrez Gene: MIP major intrinsic protein of lens fiber.