| Official Name: | Lake Timberline, Missouri |
| Settlement Type: | Census-designated place |
| Pushpin Map: | Missouri#USA |
| Pushpin Label: | Lake Timberline |
| Coordinates: | 37.9958°N -90.5456°W |
| Subdivision Type: | Country |
| Subdivision Name: | United States |
| Subdivision Type1: | State |
| Subdivision Name1: | Missouri |
| Subdivision Type2: | County |
| Subdivision Name2: | St. Francois |
| Subdivision Type3: | Townships |
| Subdivision Name3: | Big River |
| Area Footnotes: | [1] |
| Area Total Sq Mi: | 2.57 |
| Area Land Sq Mi: | 2.38 |
| Area Water Sq Mi: | 0.19 |
| Area Total Km2: | 6.65 |
| Area Land Km2: | 6.16 |
| Area Water Km2: | 0.49 |
| Population As Of: | 2020 |
| Population Density Km2: | 117.87 |
| Timezone: | Central (CST) |
| Utc Offset: | -6 |
| Timezone Dst: | CDT |
| Utc Offset Dst: | -5 |
| Elevation Ft: | 850 |
| Postal Code Type: | ZIP Code |
| Postal Code: | 63628 (Bonne Terre) |
| Area Code: | 573 |
| Blank Name: | FIPS code |
| Blank Info: | 29-40222 |
| Blank1 Name: | GNIS feature ID |
| Blank1 Info: | 2806427 |
| Population Total: | 726 |
| Unit Pref: | Imperial |
| Population Density Sq Mi: | 305.30 |
Lake Timberline is an unincorporated community and census-designated place (CDP) in St. Francois County, Missouri, United States. The community is built around several reservoirs built in the valleys of Bee Run and Primrose Creek, southwest-flowing tributaries of the Big River, which in turn flows northwest to the Meramec River.
The community is in northern St. Francois County, bordered to the north by Jefferson County. It is northwest of U.S. Route 67, 6miles north of Bonne Terre, southwest of Festus, and southwest of St. Louis.
The community was first listed as a CDP prior to the 2020 census.