Lactobacillus delbrueckii subsp. lactis explained

Lactobacillus delbrueckii subsp. lactis is a subspecies of Lactobacillus delbrueckii that is generally used to measure the amount of cobalamin in food.[1] Its growth rate is proportional to the amount of cobalamin in the growth medium. However, lactis has been demonstrated to have the option to utilize pseudocobalamin, which is inactive for humans,[2] as well as "alkali-resistant factors" (deoxyribosides and deoxynucleotides), leading to an overestimation of the amount of cobalamine in food.[3] [4] As such, new methods using HPTLC[5] or LC-MS/MS[6] have also been developed.

Notes and References

  1. The microbiological assay of vitamin B12 with Lactobacillus leichmannii. . Hoffmann CE, Stokstad EL, Hutchings B, Dornbush AC, Jukes TH . December 13, 1949. Journal of Biological Chemistry. 181. 2. 635–644 . 15393782 . 10.1016/s0021-9258(18)56584-4. 35744983 . free.
  2. Berman D, Yacowitz H, Weiser HH . A differential microbiological assay for vitamin B12 and pseudo vitamin B12 . Applied Microbiology . 4 . 1 . 49–52 . January 1956 . 13283540 . 1057156 . 10.1128/am.4.1.49-52.1956 .
  3. Miyamoto E, Kittaka-Katsura H, Adachi S, Watanabe F . Assay of vitamin B12 in edible bamboo shoots. . Vitamins . 2005 . 79 . 329-332 .
  4. Watanabe F, Schwarz J, Takenaka S, Miyamoto E, Ohishi N, Nelle E, Hochstrasser R, Yabuta Y . 6 . Characterization of vitamin B₁₂compounds in the wild edible mushrooms black trumpet (Craterellus cornucopioides) and golden chanterelle (Cantharellus cibarius) . Journal of Nutritional Science and Vitaminology . 58 . 6 . 438–441 . September 13, 2012 . 23419403 . 10.3177/jnsv.58.438 . free .
  5. Miniaturized HPTLC of Vitamin B12 Compounds in Foods . Bito T, Yabuta Y, Watanabe F . October 1, 2013. Chromatographia. 76. 19. 1333–1337 . 10.1007/s10337-013-2386-x. 97910208 .
  6. Alsberg T, Minten J, Haglund J, Törnqvist M . Determination of hydroxyalkyl derivatives of cobalamin (vitamin B12) using reversed phase high performance liquid chromatography with electrospray tandem mass spectrometry and ultraviolet diode array detection . Rapid Communications in Mass Spectrometry . 15 . 24 . 2438–2445 . December 30, 2001 . 11746915 . 10.1002/rcm.527 . 2001RCMS...15.2438A .

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