LIMS1 explained

LIM and senescent cell antigen-like-containing domain protein 1 is a protein that in humans is encoded by the LIMS1 gene.^{[1] [2]}

Function

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading.^[3]

Interactions

LIMS1 has been shown to interact with Integrin-linked kinase^[4] and NCK2.^{[2] [5]}

Further reading

• Tu Y, Li F, Wu C . Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways . Mol. Biol. Cell . 9 . 12 . 3367–82 . 1998 . 9843575 . 25640 . 10.1091/mbc.9.12.3367 .
• Scanlan MJ, Gordan JD, Williamson B, Stockert E, Bander NH, Jongeneel V, Gure AO, Jäger D, Jäger E, Knuth A, Chen YT, Old LJ . Antigens recognized by autologous antibody in patients with renal-cell carcinoma . Int. J. Cancer . 83 . 4 . 456–64 . 1999 . 10508479 . 10.1002/(SICI)1097-0215(19991112)83:4<456::AID-IJC4>3.0.CO;2-5 . 21839750 .
• Velyvis A, Yang Y, Wu C, Qin J . Solution structure of the focal adhesion adaptor PINCH LIM1 domain and characterization of its interaction with the integrin-linked kinase ankyrin repeat domain . J. Biol. Chem. . 276 . 7 . 4932–9 . 2001 . 11078733 . 10.1074/jbc.M007632200 . free .
• Tu Y, Huang Y, Zhang Y, Hua Y, Wu C . A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading . J. Cell Biol. . 153 . 3 . 585–98 . 2001 . 11331308 . 2190577 . 10.1083/jcb.153.3.585 .
• Zhang Y, Chen K, Tu Y, Velyvis A, Yang Y, Qin J, Wu C . Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites . J. Cell Sci. . 115 . Pt 24 . 4777–86 . 2002 . 12432066 . 10.1242/jcs.00166 . 13630628 .
• Campana WM, Myers RR, Rearden A . Identification of PINCH in Schwann cells and DRG neurons: shuttling and signaling after nerve injury . Glia . 41 . 3 . 213–23 . 2003 . 12528177 . 10.1002/glia.10138 . 33508275 .
• Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J . Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides . Nat. Biotechnol. . 21 . 5 . 566–9 . 2003 . 12665801 . 10.1038/nbt810 . 23783563 .
• Velyvis A, Vaynberg J, Yang Y, Vinogradova O, Zhang Y, Wu C, Qin J . Structural and functional insights into PINCH LIM4 domain-mediated integrin signaling . Nat. Struct. Biol. . 10 . 7 . 558–64 . 2003 . 12794636 . 10.1038/nsb938 . 175531 .
• Fukuda T, Chen K, Shi X, Wu C . PINCH-1 is an obligate partner of integrin-linked kinase (ILK) functioning in cell shape modulation, motility, and survival . J. Biol. Chem. . 278 . 51 . 51324–33 . 2003 . 14551191 . 10.1074/jbc.M309122200 . free.
• Bock-Marquette I, Saxena A, White MD, Dimaio JM, Srivastava D . Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair . Nature . 432 . 7016 . 466–72 . 2004 . 15565145 . 10.1038/nature03000 . 2004Natur.432..466B . 4420896 .
• Barrios-Rodiles M, Brown KR, Ozdamar B, Bose R, Liu Z, Donovan RS, Shinjo F, Liu Y, Dembowy J, Taylor IW, Luga V, Przulj N, Robinson M, Suzuki H, Hayashizaki Y, Jurisica I, Wrana JL . High-throughput mapping of a dynamic signaling network in mammalian cells . Science . 307 . 5715 . 1621–5 . 2005 . 15761153 . 10.1126/science.1105776 . 2005Sci...307.1621B . 39457788 .
• Dougherty GW, Chopp T, Qi SM, Cutler ML . The Ras suppressor Rsu-1 binds to the LIM 5 domain of the adaptor protein PINCH1 and participates in adhesion-related functions . Exp. Cell Res. . 306 . 1 . 168–79 . 2005 . 15878342 . 10.1016/j.yexcr.2005.01.025 .
• Xu Z, Fukuda T, Li Y, Zha X, Qin J, Wu C . Molecular dissection of PINCH-1 reveals a mechanism of coupling and uncoupling of cell shape modulation and survival . J. Biol. Chem. . 280 . 30 . 27631–7 . 2005 . 15941716 . 10.1074/jbc.M504189200 . free .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .

Notes and References

1. Rearden A . A new LIM protein containing an autoepitope homologous to "senescent cell antigen" . Biochem. Biophys. Res. Commun. . 201 . 3 . 1124–31 . Aug 1994 . 7517666 . 10.1006/bbrc.1994.1822 .
2. Tu Y, Li F, Goicoechea S, Wu C . The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells . Mol. Cell. Biol. . 19 . 3 . 2425–34 . Mar 1999 . 10022929 . 84035 . 10.1128/mcb.19.3.2425.
3. Web site: Entrez Gene: LIMS1 LIM and senescent cell antigen-like domains 1.
4. Zhang Y, Chen K, Guo L, Wu C . Characterization of PINCH-2, a new focal adhesion protein that regulates the PINCH-1-ILK interaction, cell spreading, and migration . J. Biol. Chem. . 277 . 41 . 38328–38 . Oct 2002 . 12167643 . 10.1074/jbc.M205576200 . free .
5. Tu Y, Li F, Wu C . Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways . Mol. Biol. Cell . 9 . 12 . 3367–82 . Dec 1998 . 9843575 . 25640 . 10.1091/mbc.9.12.3367 .