LCP1 explained

Plastin-2 is a protein that in humans is encoded by the LCP1 gene.^[1]

Function

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.^[2]

Further reading

• Namba Y, Ito M, Zu Y, Shigesada K, Maruyama K . Human T cell L-plastin bundles actin filaments in a calcium-dependent manner . Journal of Biochemistry . 112 . 4 . 503–7 . October 1992 . 1491005 . 10.1093/oxfordjournals.jbchem.a123929.
• Zu YL, Shigesada K, Nishida E, Kubota I, Kohno M, Hanaoka M, Namba Y . 65-kilodalton protein phosphorylated by interleukin 2 stimulation bears two putative actin-binding sites and two calcium-binding sites . Biochemistry . 29 . 36 . 8319–24 . September 1990 . 2252891 . 10.1021/bi00488a017 .
• Lin CS, Aebersold RH, Leavitt J . Correction of the N-terminal sequences of the human plastin isoforms by using anchored polymerase chain reaction: identification of a potential calcium-binding domain . Molecular and Cellular Biology . 10 . 4 . 1818–21 . April 1990 . 2378651 . 362293 . 10.1128/MCB.10.4.1818.
• Lin CS, Aebersold RH, Kent SB, Varma M, Leavitt J . Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts . Molecular and Cellular Biology . 8 . 11 . 4659–68 . November 1988 . 3211125 . 365555 . 10.1128/MCB.8.11.4659.
• Matsushima K, Shiroo M, Kung HF, Copeland TD . Purification and characterization of a cytosolic 65-kilodalton phosphoprotein in human leukocytes whose phosphorylation is augmented by stimulation with interleukin 1 . Biochemistry . 27 . 10 . 3765–70 . May 1988 . 3261603 . 10.1021/bi00410a037 .
• Kondo I, Shin K, Honmura S, Nakajima H, Yamamura E, Satoh H, Terauchi M, Usuki Y, Takita H, Hamaguchi H . A case report of a patient with retinoblastoma and chromosome 13q deletion: assignment of a new gene (gene for LCP1) on human chromosome 13 . Human Genetics . 71 . 3 . 263–6 . 1986 . 3864729 . 10.1007/BF00284588 . 11261385 .
• Arpin M, Friederich E, Algrain M, Vernel F, Louvard D . Functional differences between L- and T-plastin isoforms . The Journal of Cell Biology . 127 . 6 Pt 2 . 1995–2008 . December 1994 . 7806577 . 2120298 . 10.1083/jcb.127.6.1995 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . January 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Lin CS, Shen W, Chen ZP, Tu YH, Matsudaira P . Identification of I-plastin, a human fimbrin isoform expressed in intestine and kidney . Molecular and Cellular Biology . 14 . 4 . 2457–67 . April 1994 . 8139549 . 358613 . 10.1128/mcb.14.4.2457 .
• Lin CS, Park T, Chen ZP, Leavitt J . Human plastin genes. Comparative gene structure, chromosome location, and differential expression in normal and neoplastic cells . The Journal of Biological Chemistry . 268 . 4 . 2781–92 . February 1993 . 10.1016/S0021-9258(18)53842-4 . 8428952 . free .
• Lin CS, Chen ZP, Park T, Ghosh K, Leavitt J . Characterization of the human L-plastin gene promoter in normal and neoplastic cells . The Journal of Biological Chemistry . 268 . 4 . 2793–801 . February 1993 . 10.1016/S0021-9258(18)53843-6 . 8428953 . free .
• Jones SL, Brown EJ . FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils . The Journal of Biological Chemistry . 271 . 24 . 14623–30 . June 1996 . 8663066 . 10.1074/jbc.271.24.14623 . free .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . October 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Okumura Y, Yano M, Murakami M, Mori S, Towatari T, Kido H . The extracellular processing of HIV-1 envelope glycoprotein gp160 by human plasmin . FEBS Letters . 442 . 1 . 39–42 . January 1999 . 9923600 . 10.1016/S0014-5793(98)01612-3 . 40872647 . free .
• Wang J, Brown EJ . Immune complex-induced integrin activation and L-plastin phosphorylation require protein kinase A . The Journal of Biological Chemistry . 274 . 34 . 24349–56 . August 1999 . 10446213 . 10.1074/jbc.274.34.24349 . free .
• Lin CS, Lau A, Yeh CC, Chang CH, Lue TF . Upregulation of L-plastin gene by testosterone in breast and prostate cancer cells: identification of three cooperative androgen receptor-binding sequences . DNA and Cell Biology . 19 . 1 . 1–7 . January 2000 . 10668786 . 10.1089/104454900314654 .
• Jia J, Han Q, Borregaard N, Lollike K, Cygler M . Crystal structure of human grancalcin, a member of the penta-EF-hand protein family . Journal of Molecular Biology . 300 . 5 . 1271–81 . July 2000 . 10903868 . 10.1006/jmbi.2000.3925 .
• Lollike K, Johnsen AH, Durussel I, Borregaard N, Cox JA . Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner . The Journal of Biological Chemistry . 276 . 21 . 17762–9 . May 2001 . 11279160 . 10.1074/jbc.M100965200 . free .
• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y . Protein-protein interaction panel using mouse full-length cDNAs . Genome Research . 11 . 10 . 1758–65 . October 2001 . 11591653 . 311163 . 10.1101/gr.180101 .

Notes and References

1. Zu Y, Kohno M, Kubota I, Nishida E, Hanaoka M, Namba Y . Characterization of interleukin 2 stimulated 65-kilodalton phosphoprotein in human T cells . Biochemistry . 29 . 4 . 1055–62 . January 1990 . 2111166 . 10.1021/bi00456a030 .
2. Web site: Entrez Gene: LCP1 lymphocyte cytosolic protein 1 (L-plastin).