LAPC4 explained

LAPC4 cells are a cell line of human prostate cancer commonly used in the field of oncology. The tissue was harvested from the lymph node metastasis of a male patient with hormone refractory prostate cancer which was then xenografted into SCID mice and later harvested and plated on tissue culture dishes, where it can be propagated as an immortalized prostate cancer cell line.[1] [2] [3]

Characteristics

LAPC4 are a lowly adherent, epithelial cell line with high Androgen receptor and Prostate specific antigen expression.[4] Unlike the other commonly utilized, Androgen receptor positive prostate cancer cell lines LNCaP and VCaP, LAPC4 have high expression of Keratin 5, a basal marker, as well as the luminal markers Keratin 8 and Keratin 18.[4] LAPC4 also expresses mutated P53 (R175H).

The cells have an approximate doubling time of 72 hours under typical culture conditions.[5]

External links

Notes and References

  1. Klein. KA. Reiter. RE. Redula. J. Moradi. H. Zhu. XL. Brothman. AR. Lamb. DJ. Marcelli. M. Belldegrun. A. Witte. ON. Sawyers. CL. Progression of metastatic human prostate cancer to androgen independence in immunodeficient SCID mice.. Nature Medicine. April 1997. 3. 4. 402–8. 9095173. 10.1038/nm0497-402. 21273888.
  2. Book: Russell. Pamela J.. Kingsley. Elizabeth A.. Russell. Pamela J.. Jackson. Paul. Kingsley. Elizabeth A.. Prostate cancer methods and protocols. 2003. Humana Press. Totowa, N.J.. 978-1-59259-372-9. 21–39. Chapter 2: Human Prostate Cancer Cell Lines. https://www.springer.com/cda/content/document/cda_downloaddocument/9780896039780-c2.pdf?SGWID=0-0-45-434026-p173727994.
  3. Garcia. RR. Masoodi. KZ. Pascal. LE. Nelson. JB. Wang. Z. Growth of LAPC4 prostate cancer xenograft tumor is insensitive to 5α-reductase inhibitor dutasteride.. American Journal of Clinical and Experimental Urology. 2014. 2. 1. 82–91. 25374909. 4219295.
  4. van Bokhoven. A. Varella-Garcia. M. Korch. C. Johannes. WU. Smith. EE. Miller. HL. Nordeen. SK. Miller. GJ. Lucia. MS. Molecular characterization of human prostate carcinoma cell lines.. The Prostate. 1 November 2003. 57. 3. 205–25. 14518029. 10.1002/pros.10290. 32949167.
  5. Web site: Cell line LAPC-4 (CVCL_4744). Cellosaurus. 29 January 2018.

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "LAPC4".

Except where otherwise indicated, Everything.Explained.Today is © Copyright 2009-2024, A B Cryer, All Rights Reserved. Cookie policy.