LAMP3 explained

Lysosome-associated membrane glycoprotein 3 (LAMP3, Lamp3) is a protein that in humans is encoded by the LAMP3 gene.^{[1] [2]} It is one of the lysosome-associated membrane glycoproteins.

LAMP3 also known as DC-LAMP (Dendritic cell lysosomal associated membrane glycoprotein) is a member of the LAMP family along with LAMP1 and LAMP2, these proteins make up the members of the glycoconjugate coat present on the inside of the lysosomal membrane.^[3] In humans, this protein is almost exclusively found in mature Dendritic cells. While LAMP3 can be observed on the surface of dendritic cells, the protein is mainly found within lysosomes. LAMP3 first appears in the MHC Class II compartment and in cells aids in the identifying and processing of an antigen during an immune response.^[4] LAMP3 protein is linked with the maturation of dendritic cells, and as a marker for transformed type II pneumocytes or alveolar cells.^[5]

Studies have linked LAMP3 with the inhibition of the viral replication of Influenza A cells.^[6]

Structure and Tissue Distribution

LAMP3 is a Type I integral membrane protein consisting of about 416 amino acid residues with about 90% of the protein located within the lumen of the lysosomes.^[7] LAMP3 has been shown to be highly expressed in dendritic cells during cell differentiation and maturation. During human fetal development, between weeks 10 and 20, LAMP3 is highly expressed in the lungs, while in normal adult tissue cells LAMP3 is expressed in the lungs, appendix, testis and lymph nodes.^[8]

Further reading

• de Saint-Vis B, Vincent J, Vandenabeele S, etal . A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment. . Immunity . 9 . 3 . 325–36 . 1998 . 9768752 . 10.1016/S1074-7613(00)80615-9 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Akasaki K, Nakamura N, Tsukui N, etal . Human dendritic cell lysosome-associated membrane protein expressed in lung type II pneumocytes. . Arch. Biochem. Biophys. . 425 . 2 . 147–57 . 2004 . 15111122 . 10.1016/j.abb.2004.02.042 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Kanao H, Enomoto T, Kimura T, etal . Overexpression of LAMP3/TSC403/DC-LAMP promotes metastasis in uterine cervical cancer. . Cancer Res. . 65 . 19 . 8640–5 . 2005 . 16204031 . 10.1158/0008-5472.CAN-04-4112 . free .
• Kimura K, Wakamatsu A, Suzuki Y, etal . Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. . Genome Res. . 16 . 1 . 55–65 . 2006 . 16344560 . 10.1101/gr.4039406 . 1356129 .
• Elliott B, Scolyer RA, Suciu S, etal . Long-term protective effect of mature DC-LAMP+ dendritic cell accumulation in sentinel lymph nodes containing micrometastatic melanoma. . Clin. Cancer Res. . 13 . 13 . 3825–30 . 2007 . 17606713 . 10.1158/1078-0432.CCR-07-0358 . free .

Notes and References

1. Ozaki K, Nagata M, Suzuki M, Fujiwara T, Ueda K, Miyoshi Y, Takahashi E, Nakamura Y . Isolation and characterization of a novel human lung-specific gene homologous to lysosomal membrane glycoproteins 1 and 2: significantly increased expression in cancers of various tissues . Cancer Res . 58 . 16 . 3499–503 . Sep 1998 . 9721848 .
2. Web site: Entrez Gene: LAMP3 lysosomal-associated membrane protein 3.
3. Vitamin D3 regulates LAMP3 expression in monocyte derived dendritic cells. . 27697285 . 10.1016/j.cellimm.2016.09.013 . 311 . 2017 . Cell Immunol . 13–21 . Malaguarnera L, Marsullo A, Zorena K, Musumeci G, Di Rosa M.
4. Web site: ThermoFisher.
5. CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes . 14982840 . 10.1016/S0002-9440(10)63174-4 . 164 . 3 . 1613301 . 2004 . Am J Pathol . 861–71 . Salaun B, de Saint-Vis B, Pacheco N, Pacheco Y, Riesler A, Isaac S, Leroux C, Clair-Moninot V, Pin JJ, Griffith J, Treilleux I, Goddard S, Davoust J, Kleijmeer M, Lebecque S.
6. Lysosome-associated membrane glycoprotein 3 is involved in influenza A virus replication in human lung epithelial (A549) cells. 10.1186/1743-422X-8-384 . 21810281. 3162545 . 8. 2011. Virology Journal. 384. Zhou Z, Xue Q, Wan Y, Yang Y, Wang J, Hung T . free .
7. Web site: CD208 (DC-LAMP). BeckmanCoulter.
8. Web site: LAMP3 lysosomal associated membrane protein 3 [Homo sapiens (human) ]].