Laminin, alpha 5 explained

Laminin subunit alpha-5 is a protein that in humans is encoded by the LAMA5 gene.^{[1] [2]}

Function

Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.

Interactions

Laminin, alpha 5 has been shown to interact with BCAM.^{[3] [4]}

Further reading

• Utani A, Nomizu M, Yamada Y . Fibulin-2 binds to the short arms of laminin-5 and laminin-1 via conserved amino acid sequences . The Journal of Biological Chemistry . 272 . 5 . 2814–20 . Jan 1997 . 9006922 . 10.1074/jbc.272.5.2814 . free .
• Rousselle P, Keene DR, Ruggiero F, Champliaud MF, Rest M, Burgeson RE . Laminin 5 binds the NC-1 domain of type VII collagen . The Journal of Cell Biology . 138 . 3 . 719–28 . Aug 1997 . 9245798 . 2141627 . 10.1083/jcb.138.3.719 .
• Tiger CF, Champliaud MF, Pedrosa-Domellof F, Thornell LE, Ekblom P, Gullberg D . Presence of laminin alpha5 chain and lack of laminin alpha1 chain during human muscle development and in muscular dystrophies . The Journal of Biological Chemistry . 272 . 45 . 28590–5 . Nov 1997 . 9353324 . 10.1074/jbc.272.45.28590 . free .
• Mrowiec T, Melchar C, Górski A . HIV-protein-mediated alterations in T cell interactions with the extracellular matrix proteins and endothelium . Archivum Immunologiae et Therapiae Experimentalis . 45 . 2–3 . 255–9 . 1998 . 9597096 .
• Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro . DNA Research . 5 . 1 . 31–9 . Feb 1998 . 9628581 . 10.1093/dnares/5.1.31 . free .
• Shimizu H, Hosokawa H, Ninomiya H, Miner JH, Masaki T . Adhesion of cultured bovine aortic endothelial cells to laminin-1 mediated by dystroglycan . The Journal of Biological Chemistry . 274 . 17 . 11995–2000 . Apr 1999 . 10207021 . 10.1074/jbc.274.17.11995 . free . 2433/180895 . free .
• Son YJ, Scranton TW, Sunderland WJ, Baek SJ, Miner JH, Sanes JR, Carlson SS . The synaptic vesicle protein SV2 is complexed with an alpha5-containing laminin on the nerve terminal surface . The Journal of Biological Chemistry . 275 . 1 . 451–60 . Jan 2000 . 10617638 . 10.1074/jbc.275.1.451 . free .
• Köhler D, Kruse M, Stöcker W, Sterchi EE . Heterologously overexpressed, affinity-purified human meprin alpha is functionally active and cleaves components of the basement membrane in vitro . FEBS Letters . 465 . 1 . 2–7 . Jan 2000 . 10620696 . 10.1016/S0014-5793(99)01712-3 . 22895580 . free .
• Kikkawa Y, Sanzen N, Fujiwara H, Sonnenberg A, Sekiguchi K . Integrin binding specificity of laminin-10/11: laminin-10/11 are recognized by alpha 3 beta 1, alpha 6 beta 1 and alpha 6 beta 4 integrins . Journal of Cell Science . 113 (Pt 5) . 5 . 869–76 . Mar 2000 . 10.1242/jcs.113.5.869 . 10671376 .
• Champliaud MF, Virtanen I, Tiger CF, Korhonen M, Burgeson R, Gullberg D . Posttranslational modifications and beta/gamma chain associations of human laminin alpha1 and laminin alpha5 chains: purification of laminin-3 from placenta . Experimental Cell Research . 259 . 2 . 326–35 . Sep 2000 . 10964500 . 10.1006/excr.2000.4980 .
• Libby RT, Champliaud MF, Claudepierre T, Xu Y, Gibbons EP, Koch M, Burgeson RE, Hunter DD, Brunken WJ . Laminin expression in adult and developing retinae: evidence of two novel CNS laminins . The Journal of Neuroscience . 20 . 17 . 6517–28 . Sep 2000 . 10964957 . 2924637 . 10.1523/JNEUROSCI.20-17-06517.2000.
• Pierce RA, Griffin GL, Miner JH, Senior RM . Expression patterns of laminin alpha1 and alpha5 in human lung during development . American Journal of Respiratory Cell and Molecular Biology . 23 . 6 . 742–7 . Dec 2000 . 11104726 . 10.1165/ajrcmb.23.6.4202 .
• Parsons SF, Lee G, Spring FA, Willig TN, Peters LL, Gimm JA, Tanner MJ, Mohandas N, Anstee DJ, Chasis JA . Lutheran blood group glycoprotein and its newly characterized mouse homologue specifically bind alpha5 chain-containing human laminin with high affinity . Blood . 97 . 1 . 312–20 . Jan 2001 . 11133776 . 10.1182/blood.V97.1.312 . 10715366 . free .
• Saghizadeh M, Brown DJ, Castellon R, Chwa M, Huang GH, Ljubimova JY, Rosenberg S, Spirin KS, Stolitenko RB, Adachi W, Kinoshita S, Murphy G, Windsor LJ, Kenney MC, Ljubimov AV . Overexpression of matrix metalloproteinase-10 and matrix metalloproteinase-3 in human diabetic corneas: a possible mechanism of basement membrane and integrin alterations . The American Journal of Pathology . 158 . 2 . 723–34 . Feb 2001 . 11159210 . 1850323 . 10.1016/S0002-9440(10)64015-1 .
• McArthur CP, Wang Y, Heruth D, Gustafson S . Amplification of extracellular matrix and oncogenes in tat-transfected human salivary gland cell lines with expression of laminin, fibronectin, collagens I, III, IV, c-myc and p53 . Archives of Oral Biology . 46 . 6 . 545–55 . Jun 2001 . 11311202 . 10.1016/S0003-9969(01)00014-0 .
• Gagnoux-Palacios L, Allegra M, Spirito F, Pommeret O, Romero C, Ortonne JP, Meneguzzi G . The short arm of the laminin gamma2 chain plays a pivotal role in the incorporation of laminin 5 into the extracellular matrix and in cell adhesion . The Journal of Cell Biology . 153 . 4 . 835–50 . May 2001 . 11352943 . 2192378 . 10.1083/jcb.153.4.835 .
• Nagase T, Kikuno R, Ohara O . Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins . DNA Research . 8 . 4 . 179–87 . Aug 2001 . 11572484 . 10.1093/dnares/8.4.179 . free .
• Doi M, Thyboll J, Kortesmaa J, Jansson K, Iivanainen A, Parvardeh M, Timpl R, Hedin U, Swedenborg J, Tryggvason K . Recombinant human laminin-10 (alpha5beta1gamma1). Production, purification, and migration-promoting activity on vascular endothelial cells . The Journal of Biological Chemistry . 277 . 15 . 12741–8 . Apr 2002 . 11821406 . 10.1074/jbc.M111228200 . free .

Notes and References

1. Durkin ME, Loechel F, Mattei MG, Gilpin BJ, Albrechtsen R, Wewer UM . Tissue-specific expression of the human laminin alpha5-chain, and mapping of the gene to human chromosome 20q13.2-13.3 and to distal mouse chromosome 2 near the locus for the ragged (Ra) mutation . FEBS Letters . 411 . 2–3 . 296–300 . Jul 1997 . 9271224 . 10.1016/S0014-5793(97)00686-8 . 45286880 . free .
2. Web site: Entrez Gene: LAMA5 laminin, alpha 5.
3. Parsons SF, Lee G, Spring FA, Willig TN, Peters LL, Gimm JA, Tanner MJ, Mohandas N, Anstee DJ, Chasis JA . Lutheran blood group glycoprotein and its newly characterized mouse homologue specifically bind alpha5 chain-containing human laminin with high affinity . Blood . 97 . 1 . 312–20 . Jan 2001 . 11133776 . 10.1182/blood.V97.1.312 . 10715366 . free .
4. Kikkawa Y, Moulson CL, Virtanen I, Miner JH . Identification of the binding site for the Lutheran blood group glycoprotein on laminin alpha 5 through expression of chimeric laminin chains in vivo . The Journal of Biological Chemistry . 277 . 47 . 44864–9 . Nov 2002 . 12244066 . 10.1074/jbc.M208731200 . free .