L-proline amide hydrolase explained

L-proline amide hydrolase
Ec Number:	3.5.1.101

L-proline amide hydrolase (S-stereoselective piperazine-2-tert-butylcarboxamide hydrolase, LaaA, L-amino acid amidase) is an enzyme with systematic name (S)-piperidine-2-carboxamide amidohydrolase.^[1] This enzyme catalyses the following chemical reaction

(1) (S)-piperidine-2-carboxamide + H₂O

\rightleftharpoons (S)-piperidine-2-carboxylic acid + NH₃

(2) L-prolinamide + H₂O

\rightleftharpoons L-proline + NH₃

Notes and References

1. Komeda H, Harada H, Washika S, Sakamoto T, Ueda M, Asano Y . S-stereoselective piperazine-2-tert-butylcarboxamide hydrolase from Pseudomonas azotoformans IAM 1603 is a novel L-amino acid amidase . European Journal of Biochemistry . 271 . 8 . 1465–75 . April 2004 . 15066172 . 10.1111/j.1432-1033.2004.04056.x . free .