| L'Isle-sur-Serein | |
| Commune Status: | Commune |
| Image Coat Of Arms: | Blason de la ville de L'Isle-sur-Serein (Yonne).svg |
| Coordinates: | 47.5883°N 4.0053°W |
| Insee: | 89204 |
| Postal Code: | 89440 |
| Arrondissement: | Avallon |
| Canton: | Chablis |
| Mayor: | Stéphane Morel[1] |
| Term: | 2020 - 2026 |
| Elevation Min M: | 192 |
| Elevation Max M: | 298 |
| Area Km2: | 4.44 |
L'Isle-sur-Serein (in French pronounced as /lil syʁ səʁɛ̃/, literally L'Isle on Serein) is a commune in the Yonne department in Bourgogne-Franche-Comté in north-central France.