| Culuene River | |
| Pushpin Map: | Brazil |
| Pushpin Map Caption: | Mouth location in Brazil |
| River System: | Xingu River |
| Source1 Location: | Mato Grosso state |
| Mouth Coordinates: | -12.9259°N -52.8275°W |
| Subdivision Type1: | Country |
| Subdivision Name1: | Brazil |
| Length: | 600[1] |
The Culuene River, or Kuluene River is a 600 km tributary of Xingu River in Mato Grosso, a state in western Brazil. The main economic activities in the region are agriculture and cattle farming.
It joins the Xingu from the southeast in the Xingu Indigenous Park. The Culuene Biological Reserve is south of the park in the municipality of Paranatinga, Mato Grosso.