KTN1 explained

Kinectin is a protein that in humans is encoded by the KTN1 gene.^{[1] [2]}

Function

Various cellular organelles and vesicles are transported along the microtubules in the cytoplasm. Likewise, membrane recycling of the endoplasmic reticulum (ER), Golgi assembly at the microtubule organizing center, and alignment of lysosomes along microtubules are all related processes. The transport of organelles requires a special class of microtubule-associated proteins (MAPs). One of these is the molecular motor kinesin (see MIM 148760 and MIM 600025), an ATPase that moves vesicles unidirectionally toward the plus end of the microtubule. Another such MAP is kinectin, a large integral ER membrane protein. Antibodies directed against kinectin have been shown to inhibit its binding to kinesin.[supplied by OMIM]

Interactions

KTN1 has been shown to interact with EEF1D,^[3] RhoG^{[4] [5]} and RHOA.^{[4] [5] [6] [7]}

Further reading

• Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S . Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 . DNA Res. . 1 . 1 . 27–35 . 1994 . 7584026 . 10.1093/dnares/1.1.27 . free .
• Nomura N, Miyajima N, Sazuka T, Tanaka A, Kawarabayasi Y, Sato S, Nagase T, Seki N, Ishikawa K, Tabata S . Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement) . DNA Res. . 1 . 1 . 47–56 . 1994 . 7584028 . 10.1093/dnares/1.1.47 . free .
• Fütterer A, Kruppa G, Krämer B, Lemke H, Krönke M . Molecular cloning and characterization of human kinectin . Mol. Biol. Cell . 6 . 2 . 161–70 . 1995 . 7787243 . 275826 . 10.1091/mbc.6.2.161 .
• Yu H, Nicchitta CV, Kumar J, Becker M, Toyoshima I, Sheetz MP . Characterization of kinectin, a kinesin-binding protein: primary sequence and N-terminal topogenic signal analysis . Mol. Biol. Cell . 6 . 2 . 171–83 . 1995 . 7787244 . 275827 . 10.1091/mbc.6.2.171 .
• Print CG, Leung E, Harrison JE, Watson JD, Krissansen GW . Cloning of a gene encoding a human leukocyte protein characterised by extensive heptad repeats . Gene . 144 . 2 . 221–8 . 1994 . 8039706 . 10.1016/0378-1119(94)90381-6 .
• Print CG, Morris CM, Spurr NK, Rooke L, Krissansen GW . The CG-1 gene, a member of the kinectin and ES/130 family, maps to human chromosome band 14q22 . Immunogenetics . 43 . 4 . 227–9 . 1996 . 8575822 . 10.1007/BF00587304 .
• Hotta K, Tanaka K, Mino A, Kohno H, Takai Y . Interaction of the Rho family small G proteins with kinectin, an anchoring protein of kinesin motor . Biochem. Biophys. Res. Commun. . 225 . 1 . 69–74 . 1996 . 8769096 . 10.1006/bbrc.1996.1132 .
• Alberts AS, Bouquin N, Johnston LH, Treisman R . Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein beta subunits and the yeast response regulator protein Skn7 . J. Biol. Chem. . 273 . 15 . 8616–22 . 1998 . 9535835 . 10.1074/jbc.273.15.8616 . free .
• Vancoillie G, Lambert J, Mulder A, Koerten HK, Mommaas AM, Van Oostveldt P, Naeyaert JM . Kinesin and kinectin can associate with the melanosomal surface and form a link with microtubules in normal human melanocytes . J. Invest. Dermatol. . 114 . 3 . 421–9 . 2000 . 10692099 . 10.1046/j.1523-1747.2000.00896.x . free .
• Salassidis K, Bruch J, Zitzelsberger H, Lengfelder E, Kellerer AM, Bauchinger M . Translocation t(10;14)(q11.2:q22.1) fusing the kinetin to the RET gene creates a novel rearranged form (PTC8) of the RET proto-oncogene in radiation-induced childhood papillary thyroid carcinoma . Cancer Res. . 60 . 11 . 2786–9 . 2000 . 10850414 .
• Ong LL, Lim AP, Er CP, Kuznetsov SA, Yu H . Kinectin-kinesin binding domains and their effects on organelle motility . J. Biol. Chem. . 275 . 42 . 32854–60 . 2000 . 10913441 . 10.1074/jbc.M005650200 . free .
• Harrington JJ, Sherf B, Rundlett S, Jackson PD, Perry R, Cain S, Leventhal C, Thornton M, Ramachandran R, Whittington J, Lerner L, Costanzo D, McElligott K, Boozer S, Mays R, Smith E, Veloso N, Klika A, Hess J, Cothren K, Lo K, Offenbacher J, Danzig J, Ducar M . Creation of genome-wide protein expression libraries using random activation of gene expression . Nat. Biotechnol. . 19 . 5 . 440–5 . 2001 . 11329013 . 10.1038/88107 . 25064683 .
• Vignal E, Blangy A, Martin M, Gauthier-Rouvière C, Fort P . Kinectin is a key effector of RhoG microtubule-dependent cellular activity . Mol. Cell. Biol. . 21 . 23 . 8022–34 . 2001 . 11689693 . 99969 . 10.1128/MCB.21.23.8022-8034.2001 .
• Tran H, Pankov R, Tran SD, Hampton B, Burgess WH, Yamada KM . Integrin clustering induces kinectin accumulation . J. Cell Sci. . 115 . Pt 10 . 2031–40 . 2002 . 10.1242/jcs.115.10.2031 . 11973345 .
• Ong LL, Er CP, Ho A, Aung MT, Yu H . Kinectin anchors the translation elongation factor-1 delta to the endoplasmic reticulum . J. Biol. Chem. . 278 . 34 . 32115–23 . 2003 . 12773547 . 10.1074/jbc.M210917200 . free .
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G . A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway . Nat. Cell Biol. . 6 . 2 . 97–105 . 2004 . 14743216 . 10.1038/ncb1086 . 11683986 .

Notes and References

1. Rao PN, Yu H, Hodge R, Pettenati MJ, Sheetz MP . Assignment of the human kinectin gene (KTN1), encoding a kinesin-binding protein, to chromosome 14 band q22.1 by in situ hybridization . Cytogenet. Cell Genet. . 79 . 3–4 . 196–7 . 1997 . 9605849 . 10.1159/000134719 .
2. Web site: Entrez Gene: KTN1 kinectin 1 (kinesin receptor).
3. Ong LL, Er CP, Ho A, Aung MT, Yu H . Kinectin anchors the translation elongation factor-1 delta to the endoplasmic reticulum . J. Biol. Chem. . 278 . 34 . 32115–23 . Aug 2003 . 12773547 . 10.1074/jbc.M210917200 . free .
4. Neudauer CL, Joberty G, Macara IG . PIST: a novel PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10 . Biochem. Biophys. Res. Commun. . 280 . 2 . 541–7 . Jan 2001 . 11162552 . 10.1006/bbrc.2000.4160 .
5. Vignal E, Blangy A, Martin M, Gauthier-Rouvière C, Fort P . Kinectin is a key effector of RhoG microtubule-dependent cellular activity . Mol. Cell. Biol. . 21 . 23 . 8022–34 . Dec 2001 . 11689693 . 99969 . 10.1128/MCB.21.23.8022-8034.2001 .
6. Riento K, Guasch RM, Garg R, Jin B, Ridley AJ . RhoE binds to ROCK I and inhibits downstream signaling . Mol. Cell. Biol. . 23 . 12 . 4219–29 . Jun 2003 . 12773565 . 156133 . 10.1128/MCB.23.12.4219-4229.2003 .
7. Hotta K, Tanaka K, Mino A, Kohno H, Takai Y . Interaction of the Rho family small G proteins with kinectin, an anchoring protein of kinesin motor . Biochem. Biophys. Res. Commun. . 225 . 1 . 69–74 . Aug 1996 . 8769096 . 10.1006/bbrc.1996.1132 .