Krebs–Henseleit solution explained

Krebs–Henseleit solution, developed by Hans Krebs and Kurt Henseleit, is a solution containing sodium (Na), potassium (K), chloride (Cl), calcium (Ca), magnesium sulfate (MgSO₄), bicarbonate (HCO₃), phosphate (PO₄), glucose,^[1] and sometimes supplemented with albumin, and tromethamine (THAM).^[2]

It has been used experimentally, for instance to study arteries ex vivo,^[3] in Langendorff heart preparations,^[4] and during isolated muscle testing of mammalian skeletal muscles.

Notes and References

1. Krebs-Henseleit (KH) Buffer . Cold Spring Harbor Protocols . 6 May 2021 . pdb.rec581 . en . 10.1101/pdb.rec581 . 1 June 2006. 2006 .
2. http://www.curehunter.com/public/keywordSummaryC074097-Krebs-Henseleit-solution.do curehunter.com > Krebs–Henseleit solution
3. Quan A, Leung SW, Lao TT, Man RY . 5-hydroxytryptamine and thromboxane A2 as physiologic mediators of human umbilical artery closure . J. Soc. Gynecol. Investig. . 10 . 8 . 490–5 . December 2003 . 14662162 . 10.1016/S1071-5576(03)00149-7.
4. Bailey . Leslie E. . Ong . Seok D. . Krebs-Henseleit solution as a physiological buffer in perfused and superfused preparations . Journal of Pharmacological Methods . August 1978 . 1 . 2 . 171–175 . 10.1016/0160-5402(78)90022-0 . 6 May 2021.