| Knowles Brook | |
| Pushpin Map: | New York |
| Pushpin Map Caption: | Location of the mouth of Knowles Brook |
| Subdivision Type1: | Country |
| Subdivision Name1: | United States |
| Subdivision Type2: | State |
| Subdivision Name2: | New York |
| Subdivision Type4: | County |
| Subdivision Name4: | Delaware |
| Source1 Coordinates: | 41.95°N -75.1114°W |
| Mouth: | Fish Creek |
| Mouth Coordinates: | 41.9395°N -75.1238°W[1] |
| Mouth Elevation: | 1535feet |
Knowles Brook is a river in Delaware County, New York. It flows into Fish Creek east-southeast of Luzerne.