Kelling's test explained

Kelling's test is a chemical test used for detecting the presence of lactic acid in gastric juice.

Procedure

Two drops of iron(III) chloride are added to a test tube with distilled water. After mixing, it is divided into two parts. Add one millilitre of gastric juice in one test tube and the same volume of distilled water in the other test tube, which is acting as a control. The test tube with the gastric juice turns yellow in the presence of lactic acid due to the formation of ferric lactate.[1] [2] [3] [4]

Notes and References

  1. Book: Sood. Textbook of Medical Laboratory Technology. 2006. Jaypee Brothers Publishers. 978-81-8061-591-7. 444.
  2. Book: K. Bhattacharya & G.K. Chakraborty. Ganendrakumar Chakravarti. A Handbook of Clinical Pathology. 1978. Academic Publishers. 978-81-87504-85-6. 205.
  3. Book: Dandekar. Practicals And Viva In Medical Biochemistry. 1 January 2004. Elsevier India. 978-81-8147-025-6. 33.
  4. Book: Yadav. Comprehensive Practical and Viva in Biochemistry. 1 January 2003. Jaypee Brothers Publishers. 978-81-8061-210-7. 84.