| Charles William | |
| Prince of Anhalt-Zerbst | |
| House: | Ascania |
| Father: | John VI, Prince of Anhalt-Zerbst |
| Mother: | Sophie Augusta of Holstein-Gottorp |
| Spouse: | Duchess Sophia of Saxe-Weissenfels |
| Issue: | John Augustus, Prince of Anhalt-Zerbst Magdalena Augusta, Duchess of Saxe-Gotha-Altenburg |
| Birth Place: | Zerbst, Principality of Anhalt-Zerbst |
| Death Place: | Zerbst |
| Full Name: | Karl Wilhelm |
Charles William, Prince of Anhalt-Zerbst (16 October 1652 in Zerbst – 3 November 1718 in Zerbst), was a German prince of the House of Ascania and ruler of the Principality of Anhalt-Zerbst.
He was the third (but eldest surviving) son of John VI, Prince of Anhalt-Zerbst, and Sophie Auguste, daughter of Frederick III, Duke of Schleswig-Holstein-Gottorp. His two older brothers died before his birth.
Charles William succeeded his father in Anhalt-Zerbst in 1667 at the age of fifteen. During his minority, which lasted until 1674, his mother, the Dowager Princess Sophie Auguste, acted as regent.
He ordered the building of Zerbst Castle (which was made his official residence) and the St. Trinitatis Church of Zerbst, which were both inaugurated in 1696. Also, he lived many years in Jever.
In Halle on 18 June 1676 Charles William married Sophie (b. Halle, 23 June 1654 – d. Zerbst, 31 March 1724), daughter of August, Duke of Saxe-Weissenfels. They had three children :