KLC1 explained

Kinesin light chain 1 is a protein that in humans is encoded by the KLC1 gene.^{[1] [2] [3]}

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature of some of these variants has not been determined.

Interactions

KLC1 has been shown to interact with MAPK8IP3, KIF5B^{[4] [5] [6]} and KIF5A.^{[5] [6]}

Further reading

• Miki H, Okada Y, Hirokawa N . Analysis of the kinesin superfamily: insights into structure and function . Trends Cell Biol. . 15 . 9 . 467–76 . 2005 . 16084724 . 10.1016/j.tcb.2005.07.006 .
• Goedert M, Marsh S, Carter N . Localization of the human kinesin light chain gene (KNS2) to chromosome 14q32.3 by fluorescence in situ hybridization . Genomics . 32 . 1 . 173–5 . 1996 . 8786116 . 10.1006/geno.1996.0102 .
• Chernajovsky Y, Brown A, Clark J . Human kinesin light (beta) chain gene: DNA sequence and functional characterization of its promoter and first exon . DNA Cell Biol. . 15 . 11 . 965–74 . 1997 . 8945637 . 10.1089/dna.1996.15.965 .
• Khodjakov A . A specific light chain of kinesin associates with mitochondria in cultured cells . Mol. Biol. Cell . 9 . 2 . 333–43 . 1998 . 9450959 . 10.1091/mbc.9.2.333. 25259 . vanc. Lizunova EM . Minin AA . 3 . Koonce . MP . Gyoeva . FK .
• Rahman A, Friedman DS, Goldstein LS . Two kinesin light chain genes in mice. Identification and characterization of the encoded proteins . J. Biol. Chem. . 273 . 25 . 15395–403 . 1998 . 9624122 . 10.1074/jbc.273.25.15395 . free .
• Gyoeva FK, Bybikova EM, Minin AA . An isoform of kinesin light chain specific for the Golgi complex . J. Cell Sci. . 113 . 11. 2047–54 . 2000 . 10.1242/jcs.113.11.2047 . 10806115 .
• Kamal A . Axonal transport of amyloid precursor protein is mediated by direct binding to the kinesin light chain subunit of kinesin-I . Neuron . 28 . 2 . 449–59 . 2001 . 11144355 . 10.1016/S0896-6273(00)00124-0 . vanc. Stokin GB . Yang Z . 3 . Xia . Chun-Hong . Goldstein . Lawrence S.B . free.
• Verhey KJ . Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules . J. Cell Biol. . 152 . 5 . 959–70 . 2001 . 11238452 . 10.1083/jcb.152.5.959 . 2198804 . vanc. Meyer D . Deehan R . 3 . Blenis . J. . Schnapp . B. J. . Rapoport . T. A. . Margolis . B. .
• Diefenbach RJ, Diefenbach E, Douglas MW, Cunningham AL . The heavy chain of conventional kinesin interacts with the SNARE proteins SNAP25 and SNAP23 . Biochemistry . 41 . 50 . 14906–15 . 2003 . 12475239 . 10.1021/bi026417u .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M . free .
• Behrends U . Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries . Int. J. Cancer . 106 . 2 . 244–51 . 2003 . 12800201 . 10.1002/ijc.11208 . vanc. Schneider I . Rössler S . 3 . Frauenknecht . Heinrich . Golbeck . Anja . Lechner . Brigitte . Eigenstetter . Gerhard . Zobywalski . Colette . Müller-Weihrich . Stephan . free .
• McCart AE, Mahony D, Rothnagel JA . Alternatively spliced products of the human kinesin light chain 1 (KNS2) gene . Traffic . 4 . 8 . 576–80 . 2004 . 12839500 . 10.1034/j.1600-0854.2003.00113.x. free .
• Suzuki Y . Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions . Genome Res. . 14 . 9 . 1711–8 . 2004 . 15342556 . 10.1101/gr.2435604 . 515316 . vanc. Yamashita R . Shirota M . 3 . Sakakibara . Y . Chiba . J . Mizushima-Sugano . J . Nakai . K . Sugano . S .
• Dhaenens CM . Association study of three polymorphisms of kinesin light-chain 1 gene with Alzheimer's disease . Neurosci. Lett. . 368 . 3 . 290–2 . 2005 . 15364413 . 10.1016/j.neulet.2004.07.040 . vanc. Van Brussel E . Schraen-Maschke S . 3 . Pasquier . F . Delacourte . A . Sablonniere . B . 25039113 .
• Gerhard DS . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . vanc. Wagner L . Feingold EA . 3 . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
• Benzinger A . Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer . Mol. Cell. Proteomics . 4 . 6 . 785–95 . 2005 . 15778465 . 10.1074/mcp.M500021-MCP200 . vanc. Muster N . Koch HB . 3 . Yates Jr . Hermeking . H . free .
• Kimura K . Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes . Genome Res. . 16 . 1 . 55–65 . 2006 . 16344560 . 10.1101/gr.4039406 . 1356129 . vanc. Wakamatsu A . Suzuki Y . 3 . Ota . T . Nishikawa . T . Yamashita . R . Yamamoto . J . Sekine . M . Tsuritani . K .
• Pernigo S. Structural basis for kinesin-1:cargo recognition. Science. 340. 6130. 356–359. 2013. 23519214. 10.1126/science.1234264. 3693442. Lamprecht A. Steiner RA. Dodding MP. 2013Sci...340..356P.

Notes and References

1. Cabeza-Arvelaiz Y, Shih LC, Hardman N, Asselbergs F, Bilbe G, Schmitz A, White B, Siciliano MJ, Lachman LB . Cloning and genetic characterization of the human kinesin light-chain (KLC) gene . DNA Cell Biol . 12 . 10 . 881–92 . Feb 1994 . 8274221 . 10.1089/dna.1993.12.881.
2. Bowman AB, Kamal A, Ritchings BW, Philp AV, McGrail M, Gindhart JG, Goldstein LS . Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein . Cell . 103 . 4 . 583–94 . Jan 2001 . 11106729 . 10.1016/S0092-8674(00)00162-8 . free .
3. Web site: Entrez Gene: KNS2 kinesin 2.
4. Diefenbach . Russell J . Diefenbach Eve . Douglas Mark W . Cunningham Anthony L . Dec 2002 . The heavy chain of conventional kinesin interacts with the SNARE proteins SNAP25 and SNAP23 . Biochemistry . 41 . 50 . 14906–15 . 0006-2960. 12475239 . 10.1021/bi026417u .
5. Rahman . A . Friedman D S . Goldstein L S . Jun 1998 . Two kinesin light chain genes in mice. Identification and characterization of the encoded proteins . J. Biol. Chem. . 273 . 25 . 15395–403 . 0021-9258. 9624122 . 10.1074/jbc.273.25.15395 . free .
6. Rahman . A . Kamal A . Roberts E A . Goldstein L S . Sep 1999 . Defective kinesin heavy chain behavior in mouse kinesin light chain mutants . J. Cell Biol. . 146 . 6 . 1277–88 . 0021-9525. 10491391 . 10.1083/jcb.146.6.1277 . 2156125 .