KIF3A explained

Kinesin-like protein KIF3A is a protein that in humans is encoded by the KIF3A gene.^{[1] [2]}

Function

KIF3A is one subunit of the heterotrimeric motor protein, kinesin-2, that was initially isolated from sea urchin egg/embryo cytosol using microtubule affinity purification.^[3] This motor consists of two kinesin-related subunits (called KIF3A and KIF3B or 3C in vertebrates) and an associated protein (KAP3), and it transports protein complexes, nucleic acids and organelles towards the "plus" ends of microtubule tracks within cells. Work done in a broad range of eukaryotic cells has revealed that heterotrimeric kinesin-2 is the primary motor protein driving the intraflagellar transport of tubulins and other axonemal building blocks from the base of the ciliary/flagellar axoneme to their site of assembly at the distal tips.^[4] This process is required for cilium assembly/maintenance and cilium-based signalling which play key roles in various cell and developmental processes. For example, in vertebrate embryos, kinesin-2 function is required for cilia-dependent nodal flow and the development of left-right asymmetry.^[5]

Interactions

KIF3A has been shown to interact with MAP3K10.^[6]

Further reading

• Aizawa H, Sekine Y, Takemura R, etal . Kinesin family in murine central nervous system. . J. Cell Biol. . 119 . 5 . 1287–96 . 1992 . 1447303 . 10.1083/jcb.119.5.1287. 2289715 .
• Yamazaki H, Nakata T, Okada Y, Hirokawa N . KIF3A/B: a heterodimeric kinesin superfamily protein that works as a microtubule plus end-directed motor for membrane organelle transport. . J. Cell Biol. . 130 . 6 . 1387–99 . 1995 . 7559760 . 10.1083/jcb.130.6.1387. 2120571 .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery. . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Nagata K, Puls A, Futter C, etal . The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3. . EMBO J. . 17 . 1 . 149–58 . 1998 . 9427749 . 10.1093/emboj/17.1.149 . 1170366 .
• Yang Z, Goldstein LS . Characterization of the KIF3C neural kinesin-like motor from mouse. . Mol. Biol. Cell . 9 . 2 . 249–61 . 1998 . 9450952 . 10.1091/mbc.9.2.249. 25248 .
• Shimizu K, Shirataki H, Honda T, etal . Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide. . J. Biol. Chem. . 273 . 12 . 6591–4 . 1998 . 9506951 . 10.1074/jbc.273.12.6591 . free .
• Hasegawa T, Yagi A, Isobe K . Interaction between GADD34 and kinesin superfamily, KIF3A. . Biochem. Biophys. Res. Commun. . 267 . 2 . 593–6 . 2000 . 10631107 . 10.1006/bbrc.1999.1991 .
• Jimbo T, Kawasaki Y, Koyama R, etal . Identification of a link between the tumour suppressor APC and the kinesin superfamily. . Nat. Cell Biol. . 4 . 4 . 323–7 . 2002 . 11912492 . 10.1038/ncb779 . 10745049 .
• Aronov S, Aranda G, Behar L, Ginzburg I . Visualization of translated tau protein in the axons of neuronal P19 cells and characterization of tau RNP granules. . J. Cell Sci. . 115 . Pt 19 . 3817–27 . 2003 . 12235292 . 10.1242/jcs.00058 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Surpili MJ, Delben TM, Kobarg J . Identification of proteins that interact with the central coiled-coil region of the human protein kinase NEK1. . Biochemistry . 42 . 51 . 15369–76 . 2004 . 14690447 . 10.1021/bi034575v .
• Schmutz J, Martin J, Terry A, etal . The DNA sequence and comparative analysis of human chromosome 5. . Nature . 431 . 7006 . 268–74 . 2004 . 15372022 . 10.1038/nature02919 . 2004Natur.431..268S . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Brown CL, Maier KC, Stauber T, etal . Kinesin-2 is a motor for late endosomes and lysosomes. . Traffic . 6 . 12 . 1114–24 . 2006 . 16262723 . 10.1111/j.1600-0854.2005.00347.x . 25560378 . free .
• Haraguchi K, Hayashi T, Jimbo T, etal . Role of the kinesin-2 family protein, KIF3, during mitosis. . J. Biol. Chem. . 281 . 7 . 4094–9 . 2006 . 16298999 . 10.1074/jbc.M507028200 . free .
• Camargo LM, Collura V, Rain JC, etal . Disrupted in Schizophrenia 1 Interactome: evidence for the close connectivity of risk genes and a potential synaptic basis for schizophrenia. . Mol. Psychiatry . 12 . 1 . 74–86 . 2007 . 17043677 . 10.1038/sj.mp.4001880 . free .
• Pantelidou M, Zographos SE, Lederer CW, etal . Differential expression of molecular motors in the motor cortex of sporadic ALS. . Neurobiol. Dis. . 26 . 3 . 577–89 . 2007 . 17418584 . 10.1016/j.nbd.2007.02.005 . 2838469 .

Notes and References

1. Whitehead JL, Wang SY, Bost-Usinger L, Hoang E, Frazer KA, Burnside B . Photoreceptor localization of the KIF3A and KIF3B subunits of the heterotrimeric microtubule motor kinesin II in vertebrate retina . Exp. Eye Res. . 69 . 5 . 491–503 . November 1999 . 10548469 . 10.1006/exer.1999.0724 .
2. Web site: Entrez Gene: KIF3A kinesin family member 3A.
3. Cole DG, Chinn SW, Wedaman KP, Hall K, Vuong T, Scholey JM . Novel heterotrimeric kinesin-related protein purified from sea urchin eggs . Nature . 366 . 6452 . 268–70 . November 1993 . 8232586 . 10.1038/366268a0 . 1993Natur.366..268C . 4367715 .
4. Rosenbaum JL, Witman GB . Intraflagellar transport . Nat. Rev. Mol. Cell Biol. . 3 . 11 . 813–25 . November 2002 . 12415299 . 10.1038/nrm952 . 12130216 .
5. Nonaka S, Tanaka Y, Okada Y, Takeda S, Harada A, Kanai Y, Kido M, Hirokawa N . Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein . Cell . 95 . 6 . 829–37 . December 1998 . 9865700 . 10.1016/S0092-8674(00)81705-5. 62805329 . free .
6. Nagata K, Puls A, Futter C, Aspenstrom P, Schaefer E, Nakata T, Hirokawa N, Hall A . The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3 . EMBO J. . 17 . 1 . 149–58 . January 1998 . 9427749 . 1170366 . 10.1093/emboj/17.1.149 .