| Juho Matsalu | |
| Birth Date: | 22 August 1911 |
| Birth Place: | Sindi, Governorate of Livonia, Russian Empire[1] |
| Death Place: | Tartu, Estonia |
| Nationalteam1: | Estonia |
| Nationalyears1: | 1937–1940 |
| Nationalcaps1: | 14 |
| Nationalgoals1: | 0 |
Juho Matsalu (22 August 1911 - 1 August 1987) was an Estonian footballer. He played in 14 matches for the Estonia national football team from 1937 to 1940.[2] He was also named in Estonia's squad for the Group 1 qualification tournament for the 1938 FIFA World Cup.[3]