| Honorific-Prefix: | Most Reverend |
| Juan de los Barrios | |
| Archbishop of Santafé de Bogotá of the New Kingdom of Granada | |
| Church: | Catholic Church |
| Archdiocese: | Archdiocese of Santafé de Bogotá of the New Kingdom of Granada |
| Term: | 1564–1569 |
| Predecessor: | None |
| Successor: | Luis Zapata de Cárdenas |
| Consecrated By: | Juan Martínez Silíceo |
| Birth Date: | 1496 |
| Birth Place: | Villa de Peloche, Spain |
| Death Date: | February 12, 1569 (age 73) |
| Previous Post: | Bishop of Paraguay (1547–1552) Bishop of Santa Marta (1552–1564) |
| Nationality: | Spanish |
Friar Juan de los Barrios y Toledo, OFM (1496 – February 12, 1569) was a Roman Catholic prelate who served as the first Archbishop of Santafé de Bogotá of the New Kingdom of Granada, (1564–1569),[1] Bishop of Santa Marta (1552–1564),[2] and the first Bishop of Paraguay (1547–1552).[3]
Juan de los Barrios was born in Villa de Peloche, Spain and ordained a priest in the Order of Friars Minor.[4] [5] On July 1, 1547, Pope Paul III appointed him the first Bishop of Paraguay. He was consecrated by Juan Martínez Silíceo, Archbishop of Toledo. On April 2, 1552, he was appointed by Pope Julius III as Bishop of Santa Marta. On March 22, 1564, Pope Pius IV appointed him the first Archbishop of Santafé en Nueva Granada. He died on February 12, 1569.
While bishop, he was the principal consecrator of Juan de Simancas Simancas, Bishop of Cartagena and Pedro de Ágreda Sánchez Martín, Bishop of Coro.