| José Guadalupe Estudillo | |
| Birth Date: | 1838 |
| Birth Place: | San Diego, California |
| Death Date: | 1917 |
| Death Place: | San Diego, California |
| Order1: | 10th |
| Office1: | California State Treasurer |
| Term Start1: | 1875 |
| Term End1: | 1880 |
| Governor1: | Ferdinand Baehr |
| Preceded1: | John Weil |
| Office2: | County Treasurer of San Diego County |
| Term Start2: | 1864 |
| Term End2: | 1875 |
José Guadalupe Concepción Estudillo (1838-1917) was a Californio politician, who served as California State Treasurer and treasurer of San Diego County. He was a member of the prominent Estudillo family of California.
José G. Estudillo is the son of José Antonio Estudillo, a prominent early settler of San Diego, California.
He served as County treasurer during 1864 - 1875, city councilman of San Diego, and California State Treasurer, 1875–1880.
Later, he moved to Los Angeles and married Adelaide Mulholland.