| John Wellington Gwynne | |
| Honorific-Suffix: | QC |
| Office: | Puisne Justice of the Supreme Court of Canada |
| Nominator: | John A. Macdonald |
| Predecessor: | William Johnstone Ritchie |
| Successor: | David Mills |
| Term Start: | January 14, 1879 |
| Term End: | January 7, 1902 |
| Birth Date: | 30 March 1814 |
| Birth Place: | Castleknock, Ireland |
| Death Place: | Ottawa, Ontario |
| Honorific Prefix: | The Honourable |
John Wellington Gwynne, (March 30, 1814 - January 7, 1902) was a Canadian lawyer and Puisne Justice of the Supreme Court of Canada.
Born in Castleknock, Ireland, the son of William Gwynne and Eliza Nelson, he emigrated to Canada in 1832.
Gwynne was elected a bencher of the Law Society of Upper Canada in 1849 and appointed QC in 1850.
In 1874, he was appointed a puisne judge of the Ontario Court of Appeal. In 1879, he was appointed to the Supreme Court of Canada and he served until his death in 1902.