John Twisleton (c 1614–1682), of Horsmans Place, Dartford, Kent was created a baronet by the Lord Protector Oliver Cromwell. He was Sheriff of Kent.
John Twisleton was born about 1614, the son and heir of John Twisleton, of Drax and Barley, Yorkshire, and of Horsmans Place in Dartford, and Margaret, daughter of William Constable. He was admitted to Gray's Inn on 3 August 1629.
The Lord Protector Oliver Cromwell created John Twisleton a baronet on 10 April 1658, This dignity was disallowed after the Restoration in May 1660.
He was Sheriff of Kent for the year starting in 1671. He died on 4 December 1682, in his 69th year, and was buried (as "John Twisleton, Esq.") at Dartford. In the south chancel of Holy Trinity Church, Dartford, is a mural monument of white marble erected to his memory.
John Twisleton was married four times: