| John Supple | |
| Office1: | Ontario MPP |
| Term Start1: | 1867 |
| Term End1: | 1869 |
| Predecessor1: | New riding |
| Successor1: | Thomas Murray |
| Constituency1: | Renfrew North |
| Office2: | Legislative Assembly of the Province of Canada |
| Term Start2: | 1856 |
| Term End2: | 1857 |
| Predecessor2: | Francis Hincks |
| Successor2: | John Lorn McDougall Sr. |
| Constituency2: | Renfrew County, Province of Canada |
| Party: | Conservative |
| Birth Date: | 1810 |
| Birth Place: | Ireland |
| Death Place: | Pembroke, Ontario |
| Profession: | Businessman |
John Supple (ca 1810 - 1869) was an Ontario businessman and political figure. He represented Renfrew North in the 1st Parliament of Ontario as a Conservative member from 1867 to 1869.
He was born in Ireland around 1810. Supple was a lumber merchant in the Pembroke area and a director of the Upper Ottawa Steamboat Company. He was elected in an 1856 by-election to represent Renfrew in the Legislative Assembly of the Province of Canada and was elected to represent Renfrew North in the provincial legislature after Confederation. His former estate is now the site of the city hall in Pembroke.