| John Otis | |
| State: | Maine |
| Term Start: | March 4, 1849 |
| Term End: | March 3, 1851 |
| Predecessor: | Hiram Belcher |
| Successor: | Robert Goodenow |
| Office2: | Member of the Maine House of Representatives |
| Term2: | 1841, 1846–1847 |
| Office3: | Member of the Maine Senate |
| Term3: | 1842 |
| Birth Date: | 3 August 1801 |
| Birth Place: | Leeds, Massachusetts, U.S. |
| Death Place: | Hallowell, Maine, U.S. |
| Resting Place: | Hallowell Cemetery, Hallowell, Maine, U.S. |
| Party: | Whig |
| Alma Mater: | Bowdoin College |
| Profession: | Politician, lawyer |
John Otis (August 3, 1801 – October 17, 1856) was a U.S. Representative from Maine.
Born in Leeds, Massachusetts (now in Maine), Otis attended the common schools, and graduated from Bowdoin College, Brunswick, Maine, in 1823.He studied law. He was admitted to the bar and commenced practice in Hallowell, Maine, in 1826. He served as member of the State House of Representatives in 1841. He was appointed a member of the Northeastern Boundary Commission in 1842. He served in the State Senate in 1842. He was again a member of the State House of Representatives in 1846 and 1847. He was elected as a Whig to the 31st United States Congress (March 4, 1849 – March 3, 1851). He died in Hallowell, Maine, October 17, 1856. He was interred in Hallowell Cemetery.