| John Singleton Millson | |
| Image Name: | JohnMillson.jpg |
| State: | Virginia |
| District: | 2nd |
| Term Start: | March 4, 1853 |
| Term End: | March 3, 1861 |
| Preceded: | Richard K. Meade |
| Succeeded: | James H. Platt, Jr. (1870) |
| State2: | Virginia |
| District2: | 1st |
| Party: | Democratic |
| Term Start2: | March 4, 1849 |
| Term End2: | March 3, 1853 |
| Preceded2: | Archibald Atkinson |
| Succeeded2: | Thomas H. Bayly |
| Office3: | Chairman of the Committee on Revolutionary Pensions |
| Term3: | March 4, 1851 - March 3, 1853 |
| Predecessor3: | Loren P. Waldo |
| Successor3: | William Montgomery Churchwell |
| Birth Date: | 1 October 1808 |
| Birth Place: | Norfolk, Virginia |
| Death Place: | Norfolk, Virginia |
| Occupation: | Attorney |
John Singleton Millson (October 1, 1808 - March 1, 1874) was an American lawyer and politician who served six consecutive terms as a U.S. Representative from Virginia from 1849 to 1861.
Born in Norfolk, Virginia, Millson pursued an academic course.He studied law.He was admitted to the bar in 1829 and commenced practice in Norfolk.
Millson was elected as a Democrat to the Thirty-first and to the five succeeding Congresses (March 4, 1849 - March 3, 1861).He served as chairman of the Committee on Revolutionary Pensions (Thirty-second Congress).
He is notable as of one of only two Southern Democrats to have voted against the Kansas-Nebraska Act, the other being Thomas Hart Benton.
After leaving Congress. Millson resumed the practice of law.He died in Norfolk, Virginia, March 1, 1874.He was interred in Cedar Grove Cemetery.