| John Jones Clarke | |
| Order: | 1st |
| Office: | Mayor of Roxbury, Massachusetts |
| Term Start: | 1846 |
| Term End: | 1846 |
| Predecessor: | Board of Selectmen |
| Successor: | Henry A. S. Dearborn |
| Term Start2: | 1853 |
| Term End2: | 1853 |
| Term Start3: | 1836 |
| Term End3: | 1837 |
| Birth Date: | February 24, 1803 |
| Birth Place: | Norton, Massachusetts |
| Death Date: | [1] |
| Death Place: | Boston, Massachusetts |
| Nationality: | American |
| Spouse: | Rebecca Cordis Haswell |
| Children: | Mary L. Clarke (Hanson), Haswell C. Clarke |
| Alma Mater: | Norton, Framingham and Phillips Andover Academies, Harvard College. |
| Profession: | Attorney |
John Jones Clarke (February 24, 1803 – November 25, 1887) was an American politician, who served in both branches of the Massachusetts legislature and as the first Mayor of Roxbury, Massachusetts in 1846.