| John M. Holley | |
| State: | New York |
| Term Start: | March 4, 1847 |
| Term End: | March 8, 1848 |
| Predecessor: | John De Mott |
| Successor: | Esbon Blackmar |
| Office2: | Member of the New York State Assembly |
| Term Start2: | January 1, 1841 |
| Term End2: | December 31, 1841 |
| Term Start3: | January 1, 1838 |
| Term End3: | December 31, 1838 |
| Birth Name: | John Milton Holley |
| Birth Date: | 10 November 1802 |
| Birth Place: | Salisbury, Connecticut, U.S. |
| Death Place: | Jacksonville, Florida, U.S. |
| Resting Place: | Rural Cemetery, Lyons, New York, U.S. |
| Party: | Whig |
| Alma Mater: | Yale College |
| Profession: | Politician, lawyer |
John Milton Holley (November 10, 1802 – March 8, 1848) was a U.S. Representative from New York.
Born in Salisbury, Connecticut, Holley was graduated from Yale College in 1822.He studied law.He was admitted to the bar and commenced practice in Black Rock, New York, in 1825.He moved to Lyons, New York, in 1826 and continued the practice of law.He was a member of the New York State Assembly (Wayne Co.) in 1838 and 1841.He served as district attorney of Wayne County 1842–1845.He was an unsuccessful candidate for election in 1844 to the Twenty-ninth Congress.
Holley was elected as a Whig to the Thirtieth Congress and served from March 4, 1847, until his death in Jacksonville, Florida, March 8, 1848.He was interred in the Rural Cemetery, Lyons, New York.