| John Colborne Milligan | |
| Office1: | Ontario MPP |
| Term Start1: | 1923 |
| Term End1: | 1926 |
| Predecessor1: | James William McLeod |
| Successor1: | Duncan Alexander McNaughton |
| Term Start2: | 1911 |
| Term End2: | 1914 |
| Predecessor2: | William John McCart |
| Successor2: | Robert Austin Shearer |
| Constituency2: | Stormont |
| Party: | Conservative |
| Birth Date: | 11 April 1868 |
| Birth Place: | Newington, Ontario |
| Death Place: | Cornwall, Ontario |
| Occupation: | Lawyer |
John Colborne Milligan (April 11, 1868 - February 17, 1942) was an Ontario lawyer, judge and political figure. He represented Stormont in the Legislative Assembly of Ontario from 1911 to 1914 and 1923 to 1926 as a Conservative member.
He was born on a farm near Newington, Ontario, the son of William Milligan, an Irish immigrant. He taught school and then attended the University of Toronto and Osgoode Hall. He was called to the bar in 1899 and set up practice in Cornwall. Milligan served on the high school board, was a police magistrate and then juvenile court judge. In 1928, he was named King's Counsel. Milligan married Maud Percival.