John Argentine (died 1507) was an English physician who attended Edward V of England and later Arthur, Prince of Wales, and was Provost of King's College, Cambridge.
He was the son of John d'Argentine, of Great Wymondley in Hertfordshire. The Argentines had been settled in Cambridgeshire since the Norman conquest of England. He was educated at Eton and King's College, Cambridge.
Argentine was the last known attendant of the Princes in the Tower; he noted that Edward took daily confession and penance, believing that his death was near. Argentine's evidence was also the basis for French declarations that the Princes in the Tower of London had been murdered and their assassin crowned as King Richard III.
Later he became physician to Prince Arthur. He ended his life as Provost of King's College, Cambridge and is buried there in the Chantry Chapel.
Argentine is a major character in the Channel 4 drama The Princes in the Tower in which he interrogates Perkin Warbeck to test the veracity of his claim to being Richard of Shrewsbury, 1st Duke of York. He is portrayed in the show by John Castle.