| John Adolf | |
| Succession: | Duke of Holstein-Gottorp |
| Reign: | 18 October 1590 – 31 March 1616 |
| Predecessor: | Philip |
| Successor: | Frederick III |
| House: | Holstein-Gottorp, Oldenburg |
| Father: | Adolf, Duke of Holstein-Gottorp |
| Mother: | Christine of Hesse |
| Spouse: | Augusta of Denmark |
| Issue: | Frederick III, Duke of Holstein-Gottorp Elisabeth Sofie Adolf Dorothea Augusta of Schleswig-Holstein-Gottorp Hedwig Anna John X of Schleswig-Holstein-Gottorp Christian |
| Birth Date: | 27 February 1575 |
| Birth Place: | Gottorf Castle |
| Death Place: | Schleswig |
| Burial Place: | Schleswig Cathedral |
Johann Adolf of Holstein-Gottorp (27 February 1575 – 31 March 1616) was a Duke of Holstein-Gottorp.
He was a third son of Duke Adolf of Holstein-Gottorp and his wife Christine of Hesse-Kassel (or Hesse-Cassel). He became the first Lutheran Administrator of the Prince-Bishopric of Lübeck (1586–1607) and the Administrator of the Prince-Archbishopric of Bremen (1589–1596). He became the Duke after the deaths of his two elder brothers. After succeeding in 1590 his father as ruling Duke the Bremian Chapter enforced his resignation in favour of his younger brother John Frederick of Holstein-Gottorp, Prince-Bishop.
He was married on 30 August 1596 to Princess Augusta of Denmark, daughter of King Frederick II of Denmark. They had the following children:
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