| Jobst Nikolaus I, Count of Hohenzollern | |
| Noble Family: | House of Hohenzollern |
| Father: | Eitel Friedrich I, Count of Hohenzollern |
| Mother: | Ursula of Rhäzüns (died in 1477) |
| Spouse: | Agnes of Werdenberg-Heiligenberg |
| Issue: | Eitel Friedrich II Eitel Friedrich the Younger |
| Birth Date: | 1433 |
| Death Place: | Zollernalbkreis, Baden-Württemberg, Germany |
Jobst Nikolaus I, Count of Hohenzollern (also known as Jost Nikolaus I or Jos Nikolaus I; 1433 - 9 February 1488) was a German nobleman from the Swabian branch of the House of Hohenzollern. He was the ruling Count of Hohenzollern from shortly after his birth until his death.
Jobst Nikolaus I became the nominal ruler of the County of Hohenzollern shortly after his birth. Initially, he stood under the regency and guardianship of his father Eitel Friedrich I ( - 1439). Under a succession treaty of 1429 with the House of Württemberg, the county would fall to the Counts of Württemberg if the Swabian branch of the House of Hohenzollern were to die out in the male line. With Jobst Nikolaus's birth, this risk was averted.
Emperor Friedrich III granted Jobst Nikolaus I and his heirs in 1471 the right to operate a mine in his county, and the right to mint coins. In 1488, Jobst Nikolaus acquired the Lordship of Haigerloch. He rebuilt the ancestral seat, Hohenzollern Castle. It would serve as the residence of the Counts of Hohenzollern until the late 18th century.
The county had come in a precarious political situation during the reign of the preceding generation. The county was fragmented when it had been divided between his father and his uncle Friedrich XII. The financial situation was almost hopeless and there was a risk that the Swabian line of the Hohenzollerns might die out. Friedrich XII had sold some land to improve the financial situation, however, this meant that the county became politically insignificant. The situation began to improve during the reign of Jobst Nikolaus's father, Eitel Friedrich I. Jobst Nikolaus managed to enlarge the territory significantly. This positive development continued under his son Eitel Friedrich II.
In 1448, Jobst Nikolaus married Countess Agnes of Werdenberg-Heiligenberg (1434–1467), a sister of Bishop Johann II of Augsburg. Together, they had the following children: