| Jo-Hanko de Villiers | |
| Birth Date: | 28 April 1996 |
| Birth Place: | Bloemfontein, South Africa |
| School: | Hoërskool Eldoraigne |
| University: | University of Johannesburg |
| Position: | Eighth man |
| Youthyears1: | 2015–2017 |
| Years1: | 2016–2019 |
| Apps1: | 8 |
| Points1: | 30 |
| Years2: | 2017–2019 |
| Apps2: | 3 |
| Points2: | 0 |
| Years3: | 2019 |
| Apps3: | 0 |
| Points3: | 0 |
| Years4: | 2021– |
| Apps4: | 0 |
| Points4: | 0 |
| Clubupdate: | 3 March 2021 |
Jo-Hanko de Villiers (born 28 April 1996) is a South African rugby union player for the in Super Rugby, the in the Currie Cup and the in the Rugby Challenge. His regular position is eighth man.