| Country: | JPN |
| Type: | National |
| Route: | 54 |
| Map Custom: | yes |
| Length Km: | 174.5 |
| Length Ref: | [1] |
| Established: | 1 April 1963 |
| Direction A: | North |
| Terminus A: | in Matsue |
| Direction B: | South |
| Terminus B: | in Naka-ku, Hiroshima |
| Previous Type: | Route |
| Previous Route: | 53 |
| Next Type: | Route |
| Next Route: | 55 |
National Route 54 is a national highway of Japan connecting Naka-ku, Hiroshima and Matsue.
Route 54 was originally designated on 18 May 1953 as National Route 182, and this was redesignated as Route 54 when the route was promoted to a Class 1 highway.