| Country: | JPN |
| Type: | National |
| Route: | 49 |
| Map Custom: | yes |
| Length Km: | 246.4 |
| Length Ref: | [1] |
| Established: | 1 April 1963 |
| Direction A: | West |
| Terminus A: | in Chūō-ku, Niigata |
| Direction B: | East |
| Terminus B: | in Iwaki |
| Previous Type: | Route |
| Previous Route: | 48 |
| Next Type: | Route |
| Next Route: | 50 |
National Route 49 is a national highway of Japan connecting Iwaki, Fukushima and Chūō-ku, Niigata in Japan, with a total length of 246.4 km (153.11 mi).
Route 49 was designated on 18 May 1953 as National Route 115, and this was redesignated as Route 49 when the route was promoted to a Class 1 highway.