| Country: | JPN |
| Type: | National |
| Route: | 427 |
| Map Custom: | yes |
| Length Km: | 96.9 |
| Length Ref: | [1] |
| Direction A: | Northern |
| Terminus A: | Asago, Hyōgo |
| Direction B: | Southern |
| Terminus B: | Nishiwaki, Hyōgo |
| Previous Type: | Route |
| Previous Route: | 426 |
| Next Type: | Route |
| Next Route: | 428 |
National Route 427 is a national highway of Japan connecting Nishiwaki, Hyōgo and Asago, Hyōgo in Japan, with a total length of 96.9 km (60.21 mi). The southern end of the road connects with Route 175 and the northern end with Route 9.