| Country: | JPN |
| Type: | National |
| Route: | 254 |
| Map Custom: | yes |
| Length Km: | 226.1 |
| Length Ref: | [1] |
| Established: | 1 April 1963 |
| Direction A: | West |
| Terminus A: | in Matsumoto, Nagano |
| Direction B: | East |
| Terminus B: | in Bunkyō,Tokyo |
| Previous Type: | Route |
| Previous Route: | 253 |
| Next Type: | Route |
| Next Route: | 255 |
is a national highway of Japan connecting Bunkyō, Tokyo and Matsumoto, with a total length of 226.1km (140.5miles).