| Country: | JPN |
| Type: | National |
| Route: | 143 |
| Map Custom: | yes |
| Length Km: | 51 |
| Length Ref: | [1] |
| Established: | 1953 |
| Direction A: | West |
| Terminus A: | in Matsumoto, Nagano |
| Direction B: | East |
| Terminus B: | in Ueda, Nagano |
| Previous Type: | Route |
| Previous Route: | 142 |
| Next Type: | Route |
| Next Route: | 144 |
National Route 143 is a national highway of Japan connecting Matsumoto, Nagano and Ueda, Nagano in Japan, with a total length of 51km (32miles).